Fast SYBR™ Green Master Mix
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Applied Biosystems™

Fast SYBR™ Green Master Mix

Fast SYBR™ Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for자세히 알아보기
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카탈로그 번호수량
43856121 x 5 mL
43856101 x 1 mL
43856162 x 5 mL
43856175 x 5 mL
438561810 x 5 mL
43856141 x 50 mL
카탈로그 번호 4385612
제품 가격(KRW)
561,000
Each
카트에 추가하기
수량:
1 x 5 mL
제품 가격(KRW)
561,000
Each
카트에 추가하기
Fast SYBR™ Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
• Fast—Real-time PCR results in as fast as 35 minutes
• Sensitive—Detect very low copies of target
• Specific—Minimize primer-dimer and non-specific amplification
• Reproducible—Consistent amplification across a wide dynamic range Fast SYBR™ Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix. It includes the following components in an optimized buffer:
• AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
• SYBR™ Green I dye to enable detection of double-stranded DNA
• Deoxynucleotides (dNTPs) to help maintain optimal PCR results
• Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
• Passive internal reference based on proprietary ROX™ dye to enable increased precision

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we've taken the best of Fast SYBR Green Master Mix and added additional capabilities for your gene expression analysis.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(장비)7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
형식Vial
반응 수500 Reactions
패시브 참조 염료ROX (Pre-mixed)
중합효소AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
제품라인SYBR
제품 유형Real Time PCR SYBR Master Mix
순도 또는 품질 등급UP (Ultra Pure)
수량1 x 5 mL
샘플 종류DNA (Genomic), cDNA
배송 조건Dry Ice
충분500 Reactions of 20 μL Each
부피5 mL
농도2X
검출 방법SYBR
용도(애플리케이션)Gene Expression
GC-Rich PCR PerformanceHigh
라벨 또는 염료SYBR Green
PCR 방법qPCR
반응 속도Fast
Unit SizeEach
구성 및 보관
Contains 1 × 5 mL vial. The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ Fast DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components. Sufficient for 500 reactions of 20 μL each.

Store at -5 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

자주 묻는 질문(FAQ)

Is there a SYBR Green Fast Master Mix currently available?

Yes. The following reagents can be used for fast cycling: Fast SYBR Green Master Mix, EXPRESS SYBR GreenER qPCR Supermix Universal, and SYBR GreenER qPCR SuperMix for ABI PRISM instruments.

What is the synthesis rate of AmpliTaq Gold Fast DNA Polymerase LD included in the Fast SYBR Green Master Mix (Cat. No. 4385612)?

DNA synthesis rate of the AmpliTaq Gold Fast DNA Polymerase LD is 1 min/kb.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Decrease of miR-146b-5p in monocytes during obesity is associated with loss of the anti-inflammatory but not insulin signaling action of adiponectin.
Authors:Hulsmans M, Van Dooren E, Mathieu C, Holvoet P,
Journal:PLoS One
PubMed ID:22393448
Low adiponectin, a well-recognized antidiabetic adipokine, has been associated with obesity-related inflammation, oxidative stress and insulin resistance. Globular adiponectin is an important regulator of the interleukin-1 receptor-associated kinase (IRAK)/NF?B pathway in monocytes of obese subjects. It protects against inflammation and oxidative stress by inducing IRAK3. microRNA (miR)-146b-5p inhibits NF?B-mediated inflammation ... More