The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. Compared to the original AmpliTaq DNA polymerase, AmpliTaq 360 polymerase is purified by an additional proprietary separation process which reduces contaminating bacterial DNA sequences from the enzyme preparation. This ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and when combined with the proprietary AmpliTaq 360 Buffer Kit, promotes the amplification of a variety of templates including those from bacterial and human genomes.
• Optimized for the broadest range of targets—from everyday to challenging • Unmatched sensitivity and yield • Market-leading GC enhancer for robust amplification of GC-rich sequences • Achieves the highest quality sequencing data
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법
Primer-probe
형식
Stand-alone enzyme
GC-Rich PCR Performance
Medium
중합효소
AmpliTaq 360 DNA Polymerase
반응 속도
Standard
엑소뉴클레아제 활성
5' - 3'
그린 기능
Sustainable packaging
제품라인
AmpliTaq™
제품 유형
DNA Polymerase
수량
250 Units
배송 조건
Approved for shipment at Room Temperature or on Wet Ice
농도
5 U/μL
Fidelity (Taq 대비)
1 X
핫 스타트
No
반응 수
200
오버행
3'-A
반응 형식
Separate Components
크기(최종 제품)
5 kb or less
시작 물질
DNA
구성 및 보관
AmpliTaq™ 360 DNA Polymerase is supplied at 5U⁄μL with 10X AmpliTaq™ 360 PCR Buffer, 25mM M2Cl2 , and 360 GC Enhancer. Typically, 1.5mL of each of AmpliTaq 360 Buffer, 25mM MgCl2 , and 360 GC Enhancer is supplied with each 250U of enzyme. • Storage Buffer Enzyme is supplied in 20 mM Tris, pH 9.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (w⁄v) Tween 20, 50% (v⁄v) glycerol • Source: Recombinant, modified form of the Thermus aquaticus DNA polymerase gene expressed in E. coli
All solutions are prepared in deionized, filter-sterilized water. When stored at -15 to -25 °C, this product maintains performance at least until the expiration date on the tube label. Before use, thaw frozen components to room temperature and mix well.
Product is guaranteed up to 12 months as indicated by the expiration date.