Megaplex™ PreAmp Primers, Rodent Pool A
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Applied Biosystems™

Megaplex™ PreAmp Primers, Rodent Pool A

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For use when assay sensitivity is of the utmost importance, Applied Biosystems® Megaplex™ PreAmp Primers significantly enhance the ability to자세히 알아보기
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카탈로그 번호수량
439920350 reactions
카탈로그 번호 4399203
제품 가격(KRW)
1,693,000
Each
카트에 추가하기
수량:
50 reactions
제품 가격(KRW)
1,693,000
Each
카트에 추가하기
For use when assay sensitivity is of the utmost importance, Applied Biosystems® Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. With content matched to the Megaplex™ RT Primers, Megaplex™ PreAmp Primers, Rodent Pool A is designed to be used with Megaplex™ RT Primers, Rodent Pool A. Following reverse transcription using Megaplex™ RT Primers, Megaplex™ PreAmp Primers along with the TaqMan® PreAmp Master Mix uniformly amplify all microRNAs prior to quantitation by real-time PCR using TaqMan® MicroRNA Arrays.
For Research Use Only. Not for use in diagnostic procedures.
사양
Fidelity (Taq 대비)10 X
그린 기능Sustainable packaging
반응 수50 reactions
프라이머PreAmp Primers
제품라인Megaplex, TaqMan
제품 유형Preamp Primer
수량50 reactions
배송 조건Room Temperature
종Rat, Mouse
충분50 Reactions
농도10X
GC-Rich PCR PerformanceLow
PCR 방법qPCR
Unit SizeEach
구성 및 보관
Contains 150 μL of 10X primers

Store at -15° to -25°C.

자주 묻는 질문(FAQ)

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

Why can I not detect any miRNA in my sample using the TaqMan MicroRNA Assay?

Most likely, the threshold is set too high to detect miRNA in samples with low levels of expression. We recommend adjusting the threshold (Ct) or NOAMP flag threshold (Crt) to an appropriate level.

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