Megaplex™ RT Primers, Human Pool A v2.1
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Applied Biosystems™

Megaplex™ RT Primers, Human Pool A v2.1

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Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis자세히 알아보기
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카탈로그 번호수량
439996650 reactions
카탈로그 번호 4399966
제품 가격(KRW)
1,389,000
Online offer
Ends: 31-Dec-2025
1,543,000
할인액 154,000 (10%)
Each
카트에 추가하기
수량:
50 reactions
제품 가격(KRW)
1,389,000
Online offer
Ends: 31-Dec-2025
1,543,000
할인액 154,000 (10%)
Each
카트에 추가하기
Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® MicroRNA Array. Human Pool A v2.1 contains RT primers for 377 unique microRNAs and 4 controls. The microRNA targets represented in this pool tend be better characterized, more broadly expressed and⁄or expressed at a higher level compared to Human Pool B. Human Pool A v2.1 is intended for use with the TaqMan® Array, Human MicroRNA A Card v2.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.
For Research Use Only. Not for use in diagnostic procedures.
사양
그린 기능Sustainable packaging
반응 수50 Reactions
프라이머RT Primers
제품라인Megaplex, TaqMan
제품 유형RT Primer
수량50 reactions
배송 조건Room Temperature
종Human
충분50 Reactions
용도(애플리케이션)miRNA analysis
GC-Rich PCR PerformanceHigh
PCR 방법qPCR
Unit SizeEach
구성 및 보관
Store at -20°C.

자주 묻는 질문(FAQ)

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

Why can I not detect any miRNA in my sample using the TaqMan MicroRNA Assay?

Most likely, the threshold is set too high to detect miRNA in samples with low levels of expression. We recommend adjusting the threshold (Ct) or NOAMP flag threshold (Crt) to an appropriate level.

View all Megaplex™ RT Primers, Human Pool A v2.1 FAQs