Silencer™ GAPDH Positive Control siRNA, in vivo ready

Ambion™ Silencer™ GAPDH Positive Control siRNA, In Vivo Ready is ideal for developing and optimizing siRNA experiments. The control is자세히 알아보기

카탈로그 번호	수량
4404025	250 nmol

카탈로그 번호 4404025

제품 가격(KRW)

250 nmol

Ambion™ Silencer™ GAPDH Positive Control siRNA, In Vivo Ready is ideal for developing and optimizing siRNA experiments. The control is validated for use in human cell lines and optimized for mouse and rat. It is highly purified as required for animal use. 250 nmol is provided.

Validated siRNA controls for optimizing siRNA experiments
Gene-specific control siRNAs provided with scrambled, negative controls
Functionally tested in several common cell lines
HPLC purified, duplexed, and ready to use

Quality Control:

Silencer™ siRNAs are manufactured in a state-of-the-art facility under the highest quality standards. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semipermeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered and tested for the presence of endotoxin. At concentrations of 50 μM in deionized water, In Vivo Ready siRNAs contain less than 0.6 mM Na+, less than 2.0 mM K+, and less than 0.1 mM Mg²⁺. Silencer™ control siRNAs are unmodified 21-mers; they are designed to be used with Silencer™ siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion™ Silencer™ Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

For Research Use Only. Not for use in diagnostic procedures.

용도(애플리케이션)RNAi

라벨 또는 염료Unconjugated

제품라인Silencer, Ambion

제품 유형siRNA

순도HPLC

수량250 nmol

배송 조건Room Temperature

제어 유형Gene Knockdown Positive Control

RNAi TypesiRNA

TargetGAPDH

Unit SizeEach

구성 및 보관

The siRNA is provided dried down in a single tube, which should be stored at -20°C.

자주 묻는 질문(FAQ)

What controls should I use for my in vivo siRNA studies?

We offer the following in vivo siRNA controls:

Invitrogen In Vivo Negative Control #1 siRNA (Cat. No. 4457287, 5nmol; 4459405, 50 nmol; 4457289, 250 nmol)
Invitrogen In Vivo GAPDH Positive control siRNA (Cat. No. 4457288, 5 nmol; 4459407, 50 nmol, 4457291, 250 nmol)
Invitrogen In Vivo Factor VII Positive control siRNA (Cat. No. 4459408, 50 nmol; 4457292, 250 nmol)
Silencer Select Negative Control No. 1 siRNA, in vivo ready (Cat. No. 4404020, 250 nmol)
Silencer GAPDH Positive Control siRNA, in vivo ready (Cat. No. 4404025, 250 nmol)

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How stable is Stealth siRNA, can I use it for in vivo research?

Stealth siRNA is markedly more stable in serum than unmodified siRNA. This increased stability is particularly beneficial in vivo where the RNAi may be exposed to more proteases. In collaboration with Intradigm, Stealth RNAi has been demonstrated to have activity in vivo when injected intra-tumorally.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How can I order in vivo siRNA duplexes?

Pre-designed Stealth RNAi and Ambion In Vivo siRNA can be ordered directly from our pre-designed siRNA search interface (https://www.thermofisher.com/us/en/home/life-science/rnai/introduction-to-in-vivo-rnai.html?open=faq#search) to target human, mouse, or rat genes.

Custom siRNA designs for in vivo experiments can be ordered from two interfaces, depending on the product format being requested: Custom Ambion In Vivo siRNA is ordered from the GeneAssist Custom siRNA Builder (https://www.thermofisher.com/order/custom-genomic-products/tools/sirna/), while Stealth RNAi is ordered using the BLOCK-iT RNAi Express for in vivo synthetics (https://rnaidesigner.thermofisher.com/rnaiexpress/inVivoService.jsp).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Do I need endotoxin testing for my in vivo RNAi duplexes?

It is recommended to reduce the chance of eliciting an immune response.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I want to order larger scales than the standard offering, whom do I contact?

Please contact rnairesearcher@invitrogen.com regarding large scale in vivo siRNA or fill out the quote request form (www.thermofisher.com/us/en/home/global/forms/custom-sirna-mirna-quote-request.html).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

View all Silencer™ GAPDH Positive Control siRNA, in vivo ready FAQs