Applied Biosystems® PrepSEQ™ Residual DNA Sample Preparation Kit는 잔류 숙주 세포주 DNA 정량이 필요한 전형적인 샘플인 복잡한 단백질, 버퍼, 염자세히 알아보기
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4413686
100 preps
카탈로그 번호 4413686
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100 preps
Applied Biosystems® PrepSEQ™ Residual DNA Sample Preparation Kit는 잔류 숙주 세포주 DNA 정량이 필요한 전형적인 샘플인 복잡한 단백질, 버퍼, 염 혼합물에서 DNA recovery 효율을 높이도록 최적화되어 있습니다. 고농도 단백질, 낮은 pH, 높은 염 함유량을 가진 샘플을 비롯해 어려운 메트릭스에서도 DNA 정량과 recovery 일관성을 확보할 수 있습니다.
For Research Use Only. Not for use in diagnostic procedures.
Includes buffers, magnetic beads and enzymes for DNA extraction for residual DNA quantitation for 100 reactions
자주 묻는 질문(FAQ)
The magnetic beads, that are shipped with the PrepSeq Residual DNA Sample Preparation Kit (Cat. No. 4413686), are clumped together and are difficult to get back into suspension. How can I fix this?
The beads are known to clump due to precipitation of the salts in the solution. These are highly concentrated chaotropic salt solutions. The only way to get the salts and the beads back into the solution is by incubating them at 37 degrees C for at least 10 minutes or until the solution is homogenous. Intermittent high speed vortexing helps expedite the process. To get beads stuck to the lid back into solution, we recommend inverting the tube and using high speed vortexing in a pulsing manner.
If incubating at 37 degrees C does not fix the issue, we recommend incubating at 45 degrees C.
What amount of DNA can be recovered with the PrepSEQ Residual DNA Sample Preparation Kit (Cat. No. 4413686)?
With the above kit, up to 5 ng of DNA can be recovered per 30 µl magnetic beads used per reaction. PrepSEQ is specially designed to recover lower amounts of DNA (in ng to sub femto‐gram levels).