For use with VetMAX™ Reagents and VetMAX™ Controls. The VetMAX™-Plus Multiplex One-Step RT-PCR Kit is designed for multiplex amplification of자세히 알아보기
Have Questions?
카탈로그 번호
수량
4415330
100 reactions
카탈로그 번호 4415330
제품 가격(KRW)
781,000
Each
카트에 추가하기
수량:
100 reactions
제품 가격(KRW)
781,000
Each
카트에 추가하기
For use with VetMAX™ Reagents and VetMAX™ Controls. The VetMAX™-Plus Multiplex One-Step RT-PCR Kit is designed for multiplex amplification of animal pathogen RNA targets using a rapid, single-tube, real-time, reverse transcription PCR (RT-PCR) strategy. The kit includes Xeno™ RNA Control, which serves as an internal positive control for RNA purification and RT-PCR amplification. RNA is reverse-transcribed into cDNA, and then your target is amplified using AmpliTaq Gold® DNA Polymerase on a real-time PCR system.
사양
용도(장비)7500 Fast System, 7500 System
형식Tube
핫 스타트Built-In Hot Start
반응 수100 Reactions
패시브 참조 염료ROX (Pre-mixed)
중합효소AmpliTaq Gold DNA Polymerase
제품라인VetMAX
제품 유형PCR Reagents and Detection Kit for Animal Health
수량100 reactions
역전사 효소ArrayScript™ UP
샘플 종류RNA
배송 조건Dry Ice
충분100 Reactions
검출 방법Primer-probe
용도(애플리케이션)Gene Expression
형태Liquid
GC-Rich PCR PerformanceLow
PCR 방법1-step RT-qPCR
반응 속도Standard
Unit SizeEach
구성 및 보관
Store at -20°C. Includes 100 reactions with the following components: Multiplex RT-PCR Buffer, Multiplex RT-PCR Enzyme Mix, Xeno™ RNA Control, and Nuclease-free water.
자주 묻는 질문(FAQ)
What can I do to improve the sensitivity of my qPCR assay?
If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:
- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)
How do I set the baseline for my qPCR experiment?
Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).
How do I set the threshold for my qPCR experiment?
In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).
I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?
There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.
I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?
There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.