Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Applied Biosystems™

Path-ID™ Multiplex One-Step Kit

Path-ID™ Multiplex One-Step RT-PCR Kit는 멀티플렉스 정량 reverse transcription PCR (qRT-PCR)에 맞게 만들어졌습니다. 이는 TaqMan® primer⁄probe set에 여러분의 RNA 샘플을자세히 알아보기
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카탈로그 번호반응 수
4428206100 Reactions
4428207500 Reactions
44400221000 Reactions
카탈로그 번호 4428206
제품 가격(KRW)
579,000
Each
카트에 추가하기
반응 수:
100 Reactions
제품 가격(KRW)
579,000
Each
카트에 추가하기
Path-ID™ Multiplex One-Step RT-PCR Kit는 멀티플렉스 정량 reverse transcription PCR (qRT-PCR)에 맞게 만들어졌습니다. 이는 TaqMan® primer⁄probe set에 여러분의 RNA 샘플을 사용할 때 최대 4개 표적을 동시에 증폭시킵니다. RNA의 reverse transcription과 표적 증폭이 한 tube에서 한 번에 이루어집니다. 이 kit에는 Arrayscript™ reverse transcriptase와 AmpliTaq Gold® DNA Polymerase를 포함한 Multiplex Enzyme Mix와 buffer mix가 들어있습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(장비)7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT System, AB 7900 HT 384-Well System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
형식Tube
핫 스타트Built-In Hot Start
반응 수100 Reactions
패시브 참조 염료ROX (Pre-mixed)
중합효소AmpliTaq Gold DNA Polymerase
제품라인Path-ID
제품 유형One-Step RT-PCR Kit
수량100 reactions
역전사 효소ArrayScript™
샘플 종류Total RNA, Viral RNA, mRNA
충분100 Reactions
검출 방법Primer-probe
용도(애플리케이션)Pathogen Detection, Virus Detection
형태Liquid
PCR 방법1-step RT-qPCR, Multiplex qPCR
반응 속도Standard
Unit SizeEach
구성 및 보관
• 1.375 mL of 2X Multiplex RT-PCR Buffer
• 280 μL of 10X Multiplex Enzyme Mix including AmpliTaq Gold and ArrayScript
• 1.75 mL of Nuclease-free water
• Store the kit at -20°C; the Nuclease-free water may be stored at -20°C, 4°C, or room temperature

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

자주 묻는 질문(FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.