Path-ID™ Multiplex One-Step RT-PCR Kit
Path-ID™ Multiplex One-Step RT-PCR Kit
Applied Biosystems™

Path-ID™ Multiplex One-Step RT-PCR Kit

The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR). It is optimized for the자세히 알아보기
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카탈로그 번호반응 수
44421371000 Reactions
4442136500 Reactions
4442135100 Reactions
카탈로그 번호 4442137
제품 가격(KRW)
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반응 수:
1000 Reactions
The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR). It is optimized for the amplification of up to four targets simultaneously using your RNA samples and TaqMan™ primer-probe sets. Reactions are run using a single-tube, one-step procedure to reverse-transcribe the RNA and amplify your targets. The kit contains a Multiplex Enzyme Mix with Arrayscript™ reverse transcriptase and AmpliTaq Gold™ DNA Polymerase, and a buffer mix.
사양
용도(장비)7500 Fast System, 7500 System
형식Tube
핫 스타트Built-In Hot Start
반응 수1000 Reactions
패시브 참조 염료ROX (Pre-mixed)
중합효소AmpliTaq Gold DNA Polymerase
제품라인Path-ID
제품 유형Multiplex One-Step RT-PCR Kit
수량1,000 reactions
역전사 효소ArrayScript™ UP
샘플 종류RNA
충분1000 Reactions
검출 방법Primer-probe
용도(애플리케이션)Pathogen Detection, Virus Detection
GC-Rich PCR PerformanceLow
PCR 방법1-step RT-qPCR, Multiplex qPCR
반응 속도Standard
Unit SizeEach
구성 및 보관
Store at -20°C. Includes 1000 reactions with the following components: Multiplex RT-PCR Buffer, Multiplex Enzyme Mix, and Nuclease-free water.

자주 묻는 질문(FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

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