Megaplex™ RT Primers, Rodent Pool B v3.0

Applied Biosystems™

Megaplex™ RT Primers, Rodent Pool B v3.0

Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis자세히 알아보기

카탈로그 번호	수량
4444292	50 reactions

카탈로그 번호 4444292

제품 가격(KRW)

1,454,000

Ends: 31-Dec-2025

1,615,000

할인액 161,000 (10%)

카트에 추가하기

50 reactions

제품 가격(KRW)

1,454,000

Ends: 31-Dec-2025

1,615,000

할인액 161,000 (10%)

카트에 추가하기

Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® Array MicroRNA Card. Rodent Pool B v2.0 contains RT primers for 306 and 135 unique microRNAs for mouse and rat respectively plus 4 species specific controls. The microRNA targets represented in this pool tend be less well characterized, more narrowly expressed and⁄or expressed at a lower level when compared to Rodent Pool A. The pool can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

For Research Use Only. Not for use in diagnostic procedures.

그린 기능Sustainable packaging

반응 수50 Reactions

프라이머RT Primers

제품라인Megaplex, TaqMan

제품 유형RT Primer

수량50 reactions

배송 조건Room Temperature

종Rat, Mouse

충분50 Reactions

용도(애플리케이션)RT-PCR

GC-Rich PCR PerformanceLow

PCR 방법2-step RT-qPCR

Unit SizeEach

구성 및 보관

Store at -20°C.

자주 묻는 질문(FAQ)

Can Ct's greater than a cut-off be considered valid results?

Yes they can. However, it is important to recognize the true linearity and detection limits of your assay: Ct values above the cut-off can indicate non-specific amplification, unless your NTC is a true- no-target control, and you have run a statistically significant number of replicates. Any results with Ct above the recommended cut-off need to be validated with individual assays on plates.

What is a good Ct cut-off for the TaqMan MicroRNA Array Cards and TaqMan Advanced miRNA Array Cards? In other words, beyond what Ct should I not trust the data?

The typical Ct cut-off on TaqMan Array Cards is 32, which is equivalent to Ct 35 on a plate (10 µl reaction). Previous studies show that if you use pre-amplification, a Ct cut-off of 29 or 30 can be used to reduce numbers of false positives (see Technical Note Optimized protocols for human or rodent microRNA profiling with precious samples). To ensure that you have selected a correct cut-off, you should run replicates of the same sample and use Ct cut-off before you see an increase in the Standard Deviation.

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

View all Megaplex™ RT Primers, Rodent Pool B v3.0 FAQs