Applied Biosystems™

Megaplex™ Primer Pools, Rodent Pools Set v3.0

Megaplex™ Primer Pools, Rodent Pools Set v3.0 is available under a single part number containing both the Megaplex™ RT Primers자세히 알아보기

카탈로그 번호	수량
4444766	50 reactions

카탈로그 번호 4444766

제품 가격(KRW)

2,342,000

온라인 행사

Ends: 30-Jun-2026

2,602,000

할인액 260,000 (10%)

50 reactions

제품 가격(KRW)

2,342,000

온라인 행사

Ends: 30-Jun-2026

2,602,000

할인액 260,000 (10%)

Megaplex™ Primer Pools, Rodent Pools Set v3.0 is available under a single part number containing both the Megaplex™ RT Primers and Megaplex™ PreAmp Primers, Rodent Pools Sets. Megaplex™ RT Primers, Rodent Pools Set collectively contains RT primers for 641 and 373 unique microRNAs for mouse and rat respectively plus 4 species specific controls. For use when assay sensitivity is of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. The Megaplex™ Primer Pools, Rodent Pools Set is intended for use with the TaqMan® Rodent MicroRNA Array Set v3.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

For Research Use Only. Not for use in diagnostic procedures.

반응 수50 Reactions

프라이머RT Primers, PreAmp Primers

제품라인Megaplex, TaqMan

제품 유형Primer Pool

수량50 reactions

종Rat, Mouse

충분50 Reactions

용도(애플리케이션)miRNA analysis

GC-Rich PCR PerformanceLow

PCR 방법qPCR

Unit SizeEach

구성 및 보관

Store at -20°C

자주 묻는 질문(FAQ)

Can Ct's greater than a cut-off be considered valid results?

Yes they can. However, it is important to recognize the true linearity and detection limits of your assay: Ct values above the cut-off can indicate non-specific amplification, unless your NTC is a true- no-target control, and you have run a statistically significant number of replicates. Any results with Ct above the recommended cut-off need to be validated with individual assays on plates.

What is a good Ct cut-off for the TaqMan MicroRNA Array Cards and TaqMan Advanced miRNA Array Cards? In other words, beyond what Ct should I not trust the data?

The typical Ct cut-off on TaqMan Array Cards is 32, which is equivalent to Ct 35 on a plate (10 µl reaction). Previous studies show that if you use pre-amplification, a Ct cut-off of 29 or 30 can be used to reduce numbers of false positives (see Technical Note Optimized protocols for human or rodent microRNA profiling with precious samples). To ensure that you have selected a correct cut-off, you should run replicates of the same sample and use Ct cut-off before you see an increase in the Standard Deviation.

Why is water condensing on the reaction wells of the optical side of my TaqMan Array Card?

Most likely the card was not at room temperature before being removed from the packaging. Remove condensation on the reaction wells by lightly blowing room temperature pressurized nitrogen or using an air blower on the wells.
IMPORTANT: Ensure that all water condensation is removed. The optical side of the card must be free of water condensation

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

View all Megaplex™ Primer Pools, Rodent Pools Set v3.0 FAQs