TaqMan™ Array Human MicroRNA A+B Cards Set v3.0
TaqMan™ Array Human MicroRNA A+B Cards Set v3.0
Applied Biosystems™

TaqMan™ Array Human MicroRNA A+B Cards Set v3.0

TaqMan® Array Human MicroRNA Card Set v3.0은 카드 당 총 384개 TaqMan® MicroRNA Assay를 담은 2개 카드 세트입니다. 이는 754개자세히 알아보기
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카탈로그 번호수량
44449138 pack
카탈로그 번호 4444913
제품 가격(KRW)
4,921,000
Each
카트에 추가하기
수량:
8 pack
제품 가격(KRW)
4,921,000
Each
카트에 추가하기
TaqMan® Array Human MicroRNA Card Set v3.0은 카드 당 총 384개 TaqMan® MicroRNA Assay를 담은 2개 카드 세트입니다. 이는 754개 human microRNA를 5시간 내에 정확하게 정량할 수 있게 합니다. 각 array에는 데이터 정규화를 돕는 3가지 TaqMan MicroRNA Assay 내인성 대조와 인간과 관련되지 않은 TaqMan® MicroRNA Assay 하나가 음성 대조군으로 포함됩니다. 포함 assay에 대한 자세한 내용이 표적 목록(Target List) 파일에 나와있습니다. 이 array set를 사용하려면 Megaplex™ RT Primers, Human Pool Set v3.0를 구입해야 합니다. Sensitivity가 매우 중요하고 샘플이 제한된 상황에서 Megaplex™ PreAmp Primers, Human Pool Set v3.0으로 추가적인 사전 증폭 단계를 실시할 수 있습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(장비)AB 7900 HT 384-Well System
형태Dried Down
라벨 또는 염료FAM
제품라인Megaplex
수량8 pack
반응 속도Standard
연구 카테고리Gene Regulation, Epigenetics
유형Array Human MicroRNA A+B Cards Set
형식384-well Microfluidic Card
종Human
Unit SizeEach
구성 및 보관
4°C

자주 묻는 질문(FAQ)

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

Why can I not detect any miRNA in my sample using the TaqMan MicroRNA Assay?

Most likely, the threshold is set too high to detect miRNA in samples with low levels of expression. We recommend adjusting the threshold (Ct) or NOAMP flag threshold (Crt) to an appropriate level.

View all TaqMan™ Array Human MicroRNA A+B Cards Set v3.0 FAQs