POROS™ CaptureSelect™ LC Lambda Affinty HPLC Columns
POROS™ CaptureSelect™ LC Lambda Affinty HPLC Columns
Thermo Scientific™

POROS™ CaptureSelect™ LC Lambda Affinty HPLC Columns

Analytical groups supporting biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates.
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카탈로그 번호치수
44691502.1 x 30 mm
44691634.6 x 50 mm
44691684.6 x 100 mm
카탈로그 번호 4469150
제품 가격(KRW)
4,579,000
Each
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치수:
2.1 x 30 mm
제품 가격(KRW)
4,579,000
Each
카트에 추가하기

Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle.

POROS CaptureSelect LC Lambda HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands. The combination allows three minute quantitation of Fabs, immunoglobulins, and bispecific antibodies having a Lambda light chain.

Features:

  • Broad utility—POROS A, G and CaptureSelectaffinity ligands address most biological drug classes
  • Rapid—POROS resins offer fast quantitation or purification of immunoglobulins and fusion proteins
  • Hands-off operation—HPLC method can be automated for streamlined operation
  • Robust—deliver high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity

The combination of CaptureSelect LC Lambda affinity ligands when combined with POROS 20 μm resin provides a high flow rate, high performance chromatography solution for the rapid quantitation and small-scale purification of all immunoglobulins and Fabs having a Lambda light chain (all subclasses).

POROS high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 μm filtration) is required, and the eluted product is highly purified and suitable for further analysis including mass spectrometry.

Automation

POROS affinity chromatography columns are suitable for use with HPLC systems, which allows the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles

Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime results in strong re-use performance. POROS Affinity Chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

LC Lambda Column Specifications:

  • Protein Binding Capacity: 10 mg/mL
  • Species Specificity: Human
  • Column Material: PEEK™ polymer or stainless steel
  • Frit: 2 μm, titanium
  • POROS Resin: Cross-linked poly(styrenedivinylbenzene)
  • CaptureSelect Ligands: Single-domain monospecific antibody fragment produced in Saccharomyces cerevisiae
For Research Use Only. Not for use in diagnostics procedures.
사양
결합 용량10.0 mg/mL IgG
컬럼 형식Analytical Column, Biocompatible PEEK
컬럼 유형Affinity
치수2.1 x 30 mm
용리량 최대200 μL
용리량 최소100 μL
유속Up to 5000 cm/hr.
용도HPLC
고처리량 호환성Not High-throughput Compatible (Manual)
매트릭스Polystyrenedivinylbenzene
최대 압력2400 psi (170 bar)
팩킹 물질Highly Crosslinked Styrene-Divinylbenzene polymer (ST-DVB)
수량1 Column
반응성Human
샘플 종류Cell Culture Supernatants
배송 조건Wet Ice
정지상Light Chain Lambda affinity ligand, human-specific
타겟 분자Lambda Light Chain
온도2-40°C
부피(미터법)100 μL
직경(미터법)2.1 mm
길이(미터법)30 mm
물질PEEK
입자 크기20 μm
기공 크기2000 Å
제품라인POROS CaptureSelect
기술Bio-LC
pH1-10
Unit SizeEach
구성 및 보관
Column includes EZ grip fittings and column test certificate

Store at 4°C. DO NOT FREEZE.

자주 묻는 질문(FAQ)

Are POROS analytical columns compatible with HPLC systems from Waters or Agilent?

All of the pre-packed POROS columns, regardless of column size or resin, have the same 10-32 female fittings on the column. Columns are compatible with Waters and Agilent HPLC systems. You will need 1/16 inch male fittings for the HPLC to attach the column.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

For any POROS analytical column, is the column compatible with an AKTA or Avant purification system?

All of the pre-packed columns, regardless column size or resin, have the same 10-32 female fittings on the column. They are all compatible with the AKTA instrument platform, however due to the column volume of the 2.1 mmD x 30 mmL columns (0.1 mL), these will not perform well in AKTA or Avant purification systems due to the relative size of the hold- up volume in these systems. The smallest column (PEEK or SS) that could feasibly be used in these systems is the 4.6mmD x 50mmL (0.8 mL) format.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Are the pre-packed POROS CaptureSelect or any POROS analytical columns compatible with an UPLC or is the use of these columns limited to HPLC?

UPLC is typically described as chromatography with sub-2 µm particles, which drives higher efficiency/better separation independent of operating flow rate. As particle size decreases, backpressure increases so the UPLC systems tend to have higher pressure ratings compared to HPLC systems. The flow rates on UPLC systems would be sufficient for POROS columns. Pump capabilities are typically 0.1-1.0 mL/min, so not as fast as a HPLC, but sufficient. Hold-up volumes on UPLC systems are lower than HPLCs, so this can help with sensitivity and efficiency. So from a pressure and flow standpoint there should be no challenge to operate a POROS CaptureSelect column on a UPLC system.

That being said, care should be taken running POROS columns on UPLC systems. Higher pressure safety interlock MUST be set at the rated column pressure, typically 180 bar. HPLC column hardware is not rated to operate at the static pressures possible and typical on UPLC systems. A clog or the like on a UPLC system could generate unsafe pressures on any HPLC-rated column before the default UPLC safety pressure interlock is tripped.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the physical or mechanical stability of POROS resins?

The physical stability of POROS resins is high. Customers have agitated, with overhead mixing and or bottom gassing for long periods of time (5-10 hours) and have performed hundreds of diaphragm pump passes during stall packs. Keep in mind that agitation with any device that creates grinding and sheer, such as with a magnetic stir bar, is not recommended for any type of resin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should I do if a POROS column dries out or air is pumped onto the column?

POROS resins rehydrate quite well. Here is the procedure that we recommend:

1. Flow 5% ethanol over the column at ?350 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
2. If baseline noise is still present, flow 20% ethanol over the column at 300 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
3. If you have established column qualification tests and specifications, perform the tests after rewetting.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all POROS™ CaptureSelect™ LC Lambda Affinty HPLC Columns FAQs