The Ion Xpress™ Barcode Adapters 33-48 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for자세히 알아보기
Have Questions?
카탈로그 번호
수량
4474518
1 kit
카탈로그 번호 4474518
제품 가격(KRW)
-
수량:
1 kit
The Ion Xpress™ Barcode Adapters 33-48 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Barcode Adapters kits allows pooling of up to 96 fragment libraries.
Key Product Features:
• Enables multiplexing of numerous fragment library samples on a single sequencing chip with the use of robust molecular barcodes • Both sequence- and flow-optimized for equal representation all barcodes in a pool and more economical multiplexed sequencing runs • Robust error correction built in by design for added confidence in sample identification
Multiplexing with Barcode Adaptors Enables Higher Throughput Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 96 samples per run (16 with this kit), significantly decreasing the cost and handling requirements of emPCR, enrichment, and sequencing.
Sequence- and Flow-Optimized for Increased Performance and Efficiency Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool, require a minimum number of flows to interrogate, and require only 17 bases of adaptor sequencing required which results in more economical multiplexed sequencing runs.
Robust Error Correction Built In by Design Built-in error correction virtually eliminates the possibility of incorrect sample assignment.
For Research Use Only. Not for use in diagnostic procedures.
본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(장비)Ion GeneStudio™ S5 Series Sequencer, Ion Chef™ System
라이브러리Fragment Library
제품 유형Barcode Adapter Kit
수량1 kit
시퀀싱 유형Genome & DNA Sequencing
배송 조건Dry Ice
처리량96 Samples per Run
워크플로우 단계Library Barcoding
제품라인Ion Torrent
시작 물질DNA
Unit SizeEach
구성 및 보관
1 box of 17 tubes, store at -20°C.
자주 묻는 질문(FAQ)
Do libraries constructed with the Ion Xpress Barcode adapters require balancing to help ensure equal representation on the Ion PGM or Proton instrument sequencing run?
No, the Ion Xpress Barcode adapters are designed with a 3 bp adapter sequence (GAT) at the 3' end of each barcode to help minimize adapter ligation bias. When pooling barcoded libraries, accurate library quantification is extremely important to help ensure even barcode representation on the sequencing run.
How many fragment or amplicon libraries can be made per barcode with the Ion Xpress Barcode Adapters kits?
The Ion Xpress Barcode Adapters kits are available as a set of 96 (Cat. No. 4474517) or in sets of 16 (Cat. Nos.4471250, 4474009, 4474518, 4474519, 4474520 or 4474521). Each barcode kit is sufficient for preparing ? 10 libraries per barcode (10 x 16 libraries or 10 x 96 libraries) for 100 ng input, or 2 libraries per barcode for 1 µg input.
How many samples can I multiplex on a single chip for sequencing on the Ion PGM or Proton System?
The number of samples that can be multiplexed in a single sequencing run depends on the capacity of the chip, the size of the library, and the required coverage. A table of approximate capacities based on the size of the library and chip can be found in the Ion Ampliseq Preparation User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0006735_AmpliSeq_DNA_RNA_LibPrep_UG.pdf) within the section Strategies for combining Ion Ampliseq libraries.