The Ion AmpliSeq Sample ID Panel is a versatile, cost effective, and easy-to-use human SNP genotyping panel comprising 9 specially designed primer pairs that can be added to the multiplex PCR reaction to generate a unique ID during post-sequencing analysis of research samples.

Designed to advance clinical research, the Ion AmpliSeq Sample ID Panel helps reveal sample misidentification for increased confidence during data analysis and reporting. The Ion AmpliSeq Sample ID Panel can be used in conjunction with any Ion AmpliSeq ready-to-use panel or custom panel designed via the Ion AmpliSeq Designer (www.ampliseq.com).

Verify various sample combinations:
• Tumor/normal paired samples
• Samples from the same individual in longitudinal studies
• Multi-tissue or multi-tumor samples from the same individual

Sample identification through SNP genetyping
• Panel includes nine primer pairs targeting nine (female samples) or ten (male samples) validated SNPs
• SNPs are unlinked and exhibit exceptional robustness and consistently high minor allele frequency across a diverse group of human populations
• One primer pair targets the amelogenin gene and amplifies one target on X chromosome and a distinct target on Y chromosome, allowing simple and quick sample gender determination
• First letter of Sample ID code provides instant gender determination for sample
• Optimal discrimination power (∼1:5,000*) with minimal sequencing needed

Simple workflow—just one pipetting step
• Spike 1 μL of the 20X Sample ID primer pool directly into any Ion AmpliSeq primer pool for co-amplification and downstream sequencing and data analysis
• Enabled for use with any Ion Xpress barcode
• Compatible with the Ion Reporter Software, a cloud-hosted software tool for automated variant analysis

Includes:
20X Ion AmpliSeq Sample ID Primer Pool

Required:
• Torrent Suite Software (v3.0 or greater) that includes the Ion AmpliSeq Sample ID Panel plug-in
• Ion AmpliSeq Library Kit 2.0 User Guide
• Ion AmpliSeq ready-to-use panel or custom panel designed via the Ion AmpliSeq Designer (www.ampliseq.com)

¹Pakstis AJ et al. SNPs for a Universal Individual Identification Panel. Hum Genet. 2010 Mar; 127(3):315-24.
*4641 discrimination power calculations assumes no missing genotyping data