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인용 및 참조 문헌 (6)
Invitrogen™
Random Primers
Random Primers는 filter hybridization 또는 in situ hybridization을 위해 템플레이트에서 표지된 DNA 프로브 준비와 cDNA 합성 poly(A)와 상관없이 mRNA priming에자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 48190011 | 100 μL |
카탈로그 번호 48190011
제품 가격(KRW)
169,000
온라인 행사
Ends: 31-Dec-2025
187,000할인액 18,000 (10%)
Each
수량:
100 μL
제품 가격(KRW)
169,000
온라인 행사
Ends: 31-Dec-2025
187,000할인액 18,000 (10%)
Each
Random Primers는 filter hybridization 또는 in situ hybridization을 위해 템플레이트에서 표지된 DNA 프로브 준비와 cDNA 합성 poly(A)와 상관없이 mRNA priming에 사용하는 oligodeoxyribonucleotides (대부분 hexamers)입니다. 이 primer는 진정한 무작위로 DNA template에서 Klenow fragment를 사용하는 DNA 합성 또는 mRNA template에서 역전사효소를 사용하는 cDNA 합성에 적합합니다. 보관 버퍼의 간섭을 방지하기 위해 Random Primer는 저염 농도 버퍼에 제공됩니다.
성능 및 품질 검사: Random Primers 라벨링 반응에서 대조 DNA를 사용해 방사선 표지된 뉴클리오티드의 통합을 검증합니다.
성능 및 품질 검사: Random Primers 라벨링 반응에서 대조 DNA를 사용해 방사선 표지된 뉴클리오티드의 통합을 검증합니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)cDNA Synthesis
형태Liquid
제품 유형Primer
수량100 μL
배송 조건Approved for shipment on Wet or Dry Ice
농도3 μg/μL
PrimerRandom
Unit SizeEach
구성 및 보관
• Random Primers in 3 mM Tris-HCl, 0.2 mM EDTA (100 μL at 3 μg/μL)
Store at –20°C.
Guaranteed stable for 6 months when properly stored.
Store at –20°C.
Guaranteed stable for 6 months when properly stored.
자주 묻는 질문(FAQ)
Can I purchase Random Primers separately?
Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?
What is the concentration of Random Primers (Cat. No. 48190011)?
인용 및 참조 문헌 (6)
인용 및 참조 문헌
Abstract
Global Expression Profiling of Acetate-grown Escherichia coli.
Journal:J Biol Chem
PubMed ID:11815613
'This study characterized the transcript profile of Escherichia coli in acetate cultures using DNA microarray on glass slides. Glucose-grown cultures were used as a reference. At the 95% confidence level, 354 genes were up-regulated in acetate, while 370 genes were down-regulated compared with the glucose-grown culture. Generally, more metabolic genes
RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs.
Journal:Science
PubMed ID:9582122
RasGRP, a guanyl nucleotide-releasing protein for the small guanosine triphosphatase Ras, was characterized. Besides the catalytic domain, RasGRP has an atypical pair of EF hands that bind calcium and a diacylglycerol (DAG)-binding domain. RasGRP activated Ras and caused transformation in fibroblasts. A DAG analog caused sustained activation of Ras-Erk signaling
Class II histone deacetylases act as signal-responsive repressors of cardiac hypertrophy.
Journal:Cell
PubMed ID:12202037
The heart responds to stress signals by hypertrophic growth, which is accompanied by activation of the MEF2 transcription factor and reprogramming of cardiac gene expression. We show here that class II histone deacetylases (HDACs), which repress MEF2 activity, are substrates for a stress-responsive kinase specific for conserved serines that regulate
Improvements in siRNA properties mediated by 2'-deoxy-2'-fluoro-beta-D-arabinonucleic acid (FANA).
Journal:Nucleic Acids Res
PubMed ID:16554553
RNA interference (RNAi) has emerged recently as an efficient mechanism for specific gene silencing. Short double-stranded small interfering RNAs (siRNAs) are now widely used for cellular or drug target validation; however, their use for silencing clinically relevant genes in a therapeutic setting remains problematic because of their unfavourable metabolic stability
Use of an in vivo reporter assay to test for transcriptional and translational fidelity in yeast.
Journal:J Biol Chem
PubMed ID:12006589
Eukaryotic RNA polymerase II and Escherichia coli RNA polymerase possess an intrinsic ribonuclease activity that is stimulated by the polymerase-binding proteins SII and GreB, respectively. This factor-activated hydrolysis of nascent RNA has been postulated to be involved in transcription elongation as well as removal of incorrect bases misincorporated into RNA.