SMART Digest RNase Columns

Thermo Scientific SMART Digest Immobilized Ribonucleases (Rnases) Columns are designed using the same proven technology as the Thermo Scientific SMART Digest Rnase kits to provide simple, fast and highly reproducible digestion of ribonucleic acid (RNA) in a flow through format.

Obtain high-quality analytical results from mRNA digestions using SMART Digest RNase columns. A significant advance in sample preparation for oligonucleotide mapping, the columns provide fast, simple digestion with high levels of reproducibility, RNase columns use immobilized ribonucleases which allow control of partial digestion to produce required fragments of ideal length for sequencing by HRAM-MS.

Easy and simple

• Simple process allows control of digestion time ensuring required fragment length for comprehensive mRNA sequencing by HRAM MS
• Single nuclease digestion allows full characterization of mRNA

Reliable results

• Increased reproducibility over existing protocols
• Higher data confidence
• No nuclease contamination of the analytical column

RNase columns are available with two enzyme specificities:

• RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues
• RNase A is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues