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Thermo Scientific™
SMART Digest™ Dual Enzyme Columns for HDX-MS Analysis
Thermo Scientific™ SMART Digest™ Dual Enzyme Columns for HDX-MS analysis provide fast, reliable digestion of proteins for HDX-MS analysis, helping to provide the sequence characterization of biotherapeutics.
| 카탈로그 번호 | 입자 크기 |
|---|---|
| 60160-110 | ˜20 μm |
카탈로그 번호 60160-110
제품 가격(KRW)
-
입자 크기:
˜20 μm
Thermo Scientific SMART Digest Dual Enzyme columns containing pepsin and protease XIII provide fast, reliable digestion of proteins for for HDX-MS analysis helping to provide the sequence characterization of biotherapeutics.
SMART Digest Dual Enzyme columns for HDX-MS analysis help to provide the characterization of biotherapeutics.
The combination of enzymes are complementary, creating many short, overlapping N-terminal peptides for a high degree of sequence coverage.
Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. The optimum pH range for pepsin activity is 1.0–3.0.
Proteinase XIII cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
사양
컬럼 형식Immobilized enzyme
컬럼 유형Immobilized enzyme in PEEK cartridge
설명SMART Digest Pepsin/Protease XIII Dual Enzyme Column
직경2.1 mm
유속10 to 500 μL/min
용도(애플리케이션)HDX-MS analysis
길이30 mm
최대 압력2500 psi (170 bar)
이동상 호환성Phosphate buffer pH 4
작동 온도 범위4 °C to 50 °C
팩킹 물질Hydrophilic/coated DVB
입자 크기˜20 μm
기공 크기° 1000 angstroms
안정성1 year
정지상Immobilized enzyme
보관 온도RT for up to 2 weeks; 4 °C for extended period (refrigerate in MES pH 6)
기술BioLC
무게8 g
CleavagePepsin: Cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. The optimum pH range for pepsin activity is 1.0–3.0.
Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
효소Pepsin & Protease XIII
유형SMART Digest Pepsin/Protease XIII Dual Enzyme column
pH2 to 6
Unit SizeEach