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인용 및 참조 문헌 (1)
Gibco™
MEM, powder
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used자세히 알아보기
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보기 방식 변경
| 카탈로그 번호 | 수량 |
|---|---|
| 61100103 | 50 L |
| 61100061 | 10 x 1 L |
| 61100087 | 10 L |
카탈로그 번호 61100103
제품 가격(KRW)
225,000
Each
수량:
50 L
제품 가격(KRW)
225,000
Each
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law..
cGMP Manufacturing and Quality System
Gibco™ MEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco™ MEM product made in our Scotland facility (61100-053). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
This product is not used for in vitro diagnostic purpose in some countries.
This MEM is modified as follows:
| With | Without |
| • L-glutamine | • HEPES |
| • Phenol Red | • Sodium Bicarbonate |
The complete formulation is available.
Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law..
cGMP Manufacturing and Quality System
Gibco™ MEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco™ MEM product made in our Scotland facility (61100-053). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
This product is not used for in vitro diagnostic purpose in some countries.
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
사양
세포주HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
세포 유형Primary Rat Astrocytes
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형MEM (Minimum Essential Medium)
수량50 L
유통 기한36 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin-free
형태Powder
Serum LevelStandard Serum Supplementation
멸균Sterile-filtered
첨가제 포함Low Glucose, Glutamine, Phenol Red
첨가제 없음No HEPES, No Sodium Pyruvate, No Sodium Bicarbonate
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
자주 묻는 질문(FAQ)
Where can I find the osmolality for MEM Medium?
What is the shelf life of my powdered media once I reconstitute it?
How long can I keep my media after supplementing with serum?
My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
How can I remove mycoplasma contamination from my cell culture medium?
인용 및 참조 문헌 (1)
인용 및 참조 문헌
Abstract
Hsp105alpha suppresses the aggregation of truncated androgen receptor with expanded CAG repeats and cell toxicity.
Journal:J Biol Chem
PubMed ID:12714591
Spinal and bulbar muscular atrophy (SBMA) is a neurodegenerative disorder caused by the expansion of a polyglutamine tract in the androgen receptor (AR). The N-terminal fragment of AR containing the expanded polyglutamine tract aggregates in cytoplasm and/or in nucleus and induces cell death. Some chaperones such as Hsp40 and Hsp70