CaptureSelect™ HRP Anti-C-tag Conjugate

CaptureSelect HRP Anti-C-tag Conjugate consists of a 13-kDa recombinant single-domain antibody fragment (VHH affinity ligand) with high affinity and selectivity for the four-amino-acid 'C-tag' peptide tag E‐P‐E‐A (glutamic acid-proline-glutamic acid-alanine). The anti-C-tag affinity ligand recognizes the E-P-E-A peptide when this tag is fused directly to the C-terminus of a protein. The affinity ligand is chemically conjugated to horseradish peroxidase (HRP) via an appropriate spacer that retains the binding reactivity of the ligand.

Applications for CaptureSelect HRP Anti-C-tag Conjugate include detection of C-tag fusion proteins in direct ELISA or western blot applications.

• Format type: HRP, liquid
• Buffer: PBS, 50% (v/v) glycerol
• Storage: in freezer (-5 to -30°C)
• Concentration: 0.5 mg/mL
• Volume: 0.1 mL
• Recommended working dilution for ELISA and western blot: 1:1000 to 1:5000 (the optimal working dilution should be determined empirically for each application)

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12- to 15-kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).