CaptureSelect™ HRP Anti-CD63 Conjugate

CaptureSelect HRP Anti-CD63 Conjugate includes a 12–15-kDa recombinant single-domain antibody fragment (VHH affinity ligand) that specifically binds with high affinity to the tetraspanin marker CD63. The affinity ligand is chemically conjugated to horseradish peroxidase (HRP) via an appropriate spacer to maintain full binding capacity. This conjugate allows you to detect, quantitate, and characterize CD63-expressing extracellular vesicles (EVs) like exosomes.

CaptureSelect HRP Anti-CD63 Conjugate can be combined with CaptureSelect Biotin Anti-CD63 Conjugate (Cat. No. 7103992100) in enzyme-linked immunoassays to quantify the CD63-expressing EVs. Sandwich ELISA setups can be designed using the biotin anti-CD63 conjugate as a capture reagent immobilized on a streptavidin-coated surface. HRP anti-CD63 conjugate is then used for detection of bound CD63-positive EVs.

For detection of a broad variety of EVs and exosomes that originate from different cell types and secretion pathways, such as those of endosomal (CD63-enriched, CD9+, or CD81+) or ectosomal (CD63 low, CD9+, and CD81+) origin, we also offer CaptureSelect Biotin and HRP anti-CD81 conjugates and CaptureSelect Biotin and HRP anti-CD9 conjugates.

Recommended working dilution for sandwich ELISA: 1:5000 to 1:10,000 (the optimal working dilution should be determined empirically for each application).