Mammalian beta-Galactosidase Assay Kit
Mammalian beta-Galactosidase Assay Kit
Thermo Scientific™

Mammalian beta-Galactosidase Assay Kit

The Thermo Scientific Mammalian β-Galactosidase Assay Kit provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal자세히 알아보기
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카탈로그 번호수량
7570775 mL
카탈로그 번호 75707
제품 가격(KRW)
374,000
온라인 행사
Ends: 31-Dec-2025
439,000
할인액 65,000 (15%)
Each
카트에 추가하기
수량:
75 mL
제품 가격(KRW)
374,000
온라인 행사
Ends: 31-Dec-2025
439,000
할인액 65,000 (15%)
Each
카트에 추가하기
The Thermo Scientific Mammalian β-Galactosidase Assay Kit provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.

Features of the Mammalian β-Galactosidase Assay Kit:

• Choose stand-alone assay reagent or complete kit for cell lysis and detection
• Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm)
• Adaptable to 96-well microplates or tube assay formats
• Perform homogeneous screening assays or measure activity secondarily after cell lysis
• Also compatiible with prepared lysates from other species such as bacteria

The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Related Products
beta-Galactosidase Assay Reagent
beta-Galactosidase Assay Stop Solution
For Research Use Only. Not for use in diagnostic procedures.
사양
설명Pierce Mammalian beta-Galactosidase Assay Kit
검출 방법Reporter Enzyme
염료 유형Enzyme Labeled
형식96-well Plate
수량75 mL
기질Proprietary
기질 유형Beta-Gal Substrate
충분250 microplate wells of adherent cells
타겟Beta-Galactosidase
용도(애플리케이션)Absorbance
용도 (장비)Microplate Reader
제품라인Pierce
제품 유형Beta-Galactosidase Assay Kit
Unit SizeEach
구성 및 보관
Sufficient For: 250 microplate wells of adherent cells
  • Pierce beta-Galactosidase Assay Reagent, 25 mL
  • M-PER Mammalian Protein Extraction Reagent, 25 mL
  • Stop Solution, 25 mL

    Store the β-Galactosidase Assay Reagent at -20°C. Store all other components at room temperature.
  • 자주 묻는 질문(FAQ)

    I tested the Mammalian beta-Galactosidase Assay Kit and did not use the Stop Solution. I performed three absorbance readings and the values were different, why is this?

    Using the Assay Stop Solution is optional. However, if the Stop Solution is not used, the absorbance values from the plate will change over time. This means that the absorbance needs to be read immediately after the 30 min incubation.

    Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

    How do I prepare cells for the Mammalian beta-Galactosidase Assay Kit?

    Thermo Scientific M-PER Mammalian Protein Extraction Reagent (Cat. No.78503) may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate can be used to assay for beta-galactosidase activity. 

    Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.