Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
인용 및 참조 문헌 (5)
Thermo Scientific™

Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)

Thermo Scientific Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X)은 단일 용액의 편리함과 세포와 조직 용해물을 위한 전체 단백질 샘플자세히 알아보기
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카탈로그 번호수량
784411 mL
7844324 x 100 μL
784455 x 1 mL
7844710 mL
카탈로그 번호 78441
제품 가격(KRW)
377,000
Online offer
Ends: 31-Dec-2025
418,000
할인액 41,000 (10%)
Each
카트에 추가하기
수량:
1 mL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
377,000
Online offer
Ends: 31-Dec-2025
418,000
할인액 41,000 (10%)
Each
카트에 추가하기
Thermo Scientific Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X)은 단일 용액의 편리함과 세포와 조직 용해물을 위한 전체 단백질 샘플 보호 기능을 제공합니다.

Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X)의 특징:

• 완벽한 보호—all-in-one 칵테일은 단백질분해효소와 phosphatase 억제제를 함유
• 여러 패키지 크기—거의 모든 규모에 잘 맞는 편리한 4가지 패키지 크기 중에서 선택 가능
• 편의성 —100배 농축 액체 제형은 개별 억제제 및 다른 형식보다 사용하기에 훨씬 편리하고 효과적임
• 쉬운 보관—수성 형식이므로 DMSO 기반 칵테일과 달리 동결하지 않고 편리하게 저온 보관 가능
• 호환성—피크 전이를 유발할 수 있는 AEBSF를 함유하지 않기 때문에 질량분광분석법(MS)에서 사용 가능
• 검증 완료—Thermo Scientific Pierce Cell Lysis Buffer에서 완벽하게 사용 가능

Halt Protease and Phosphatase Inhibitor Cocktail은 특히 추출과 정제 중에 단백질이 분해되지 않도록 최적화되어 있습니다. 이 칵테일은 주요 등급의 단백질분해효소와 phosphatase에 대한 억제제를 함유하고 있어 aminopeptidase, cysteine 및 serine protease, serine/threonine 및 protein tyrosine phosphatase를 표적화합니다. metalloprotease 억제를 촉진할 수 있도록 별도의 튜브에 0.5M EDTA가 들어 있습니다.

관련 자료:
단백질분해효소 및 phosphatase 억제제 검토

관련 제품:
Protease and Phosphatase Inhibitor Cocktail 및 Tablet 전체
For Research Use Only. Not for use in diagnostic procedures.
사양
버퍼Lysis Buffer
설명Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-Free
억제제Protease Inhibitor, Phosphatase Inhibitor
수량1 mL
시약 유형Protease Inhibitor, Phosphatase Inhibitor
형태Liquid
제품라인Halt
제품 유형Protease and Phosphatase Inhibitor Cocktail
Unit SizeEach
구성 및 보관
Upon receipt store at 4°C. Product shipped with an ice pack. Do not freeze product.

자주 묻는 질문(FAQ)

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X), 1 mL FAQs

인용 및 참조 문헌 (5)

인용 및 참조 문헌
Abstract
Olaratumab Exerts Antitumor Activity in Preclinical Models of Pediatric Bone and Soft Tissue Tumors through Inhibition of Platelet-Derived Growth Factor Receptor α.
Authors:Lowery CD,Blosser W,Dowless M,Knoche S,Stephens J,Li H,Surguladze D,Loizos N,Luffer-Atlas D,Oakley GJ 3rd,Guo Q,Iyer S,Rubin BP,Stancato L
Journal:Clinical cancer research : an official journal of the American Association for Cancer Research
PubMed ID:29191969
Purpose: Platelet-derived growth factor receptor α (PDGFRα) is implicated in several adult and pediatric malignancies, where activated signaling in tumor cells and/or cells within the microenvironment drive tumorigenesis and disease progression. Olaratumab (LY3012207/IMC-3G3) is a human mAb that exclusively binds to PDGFRα and recently received accelerated FDA approval and conditional ... More
Reverse phase protein microarray technology in traumatic brain injury.
Authors:Gyorgy AB,Walker J,Wingo D,Eidelman O,Pollard HB,Molnar A,Agoston DV
Journal:Journal of neuroscience methods
PubMed ID:20674607
Antibody based, high throughput proteomics technology represents an exciting new approach in understanding the pathobiologies of complex disorders such as cancer, stroke and traumatic brain injury. Reverse phase protein microarray (RPPA) can complement the classical methods based on mass spectrometry as a high throughput validation and quantification method. RPPA technology ... More
Distinct roles for Notch1 and Notch3 in human adipose-derived stem/stromal cell adipogenesis.
Authors:Liu MC,Logan H,Newman JJ
Journal:Molecular biology reports
PubMed ID:33021719
The role of the Notch signaling pathway in adipogenesis has long been controversial as the action of individual Notch receptors appears to vary with experimental conditions. In this study, we offer some explanation for the observed contradictions by comparing the role of both Notch1 and Notch3 in regulating the expression ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More
The Src inhibitor dasatinib accelerates the differentiation of human bone marrow-derived mesenchymal stromal cells into osteoblasts.
Authors:Id Boufker H, Lagneaux L, Najar M, Piccart M, Ghanem G, Body JJ, Journé F
Journal:BMC Cancer
PubMed ID:20565769
The proto-oncogene Src is an important non-receptor protein tyrosine kinase involved in signaling pathways that control cell adhesion, growth, migration and differentiation. It negatively regulates osteoblast activity, and, as such, its inhibition is a potential means to prevent bone loss. Dasatinib is a new dual Src/Bcr-Abl tyrosine kinase inhibitor initially ... More