Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
인용 및 참조 문헌 (5)
Thermo Scientific™

Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)

Thermo Scientific Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X)은 단일 용액의 편리함과 세포와 조직 용해물을 위한 전체 단백질 샘플자세히 알아보기
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카탈로그 번호수량
7844324 x 100 μL
784411 mL
784455 x 1 mL
7844710 mL
카탈로그 번호 78443
제품 가격(KRW)
510,000
Online offer
Ends: 31-Dec-2025
566,000
할인액 56,000 (10%)
Each
카트에 추가하기
수량:
24 x 100 μL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
510,000
Online offer
Ends: 31-Dec-2025
566,000
할인액 56,000 (10%)
Each
카트에 추가하기
Thermo Scientific Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X)은 단일 용액의 편리함과 세포와 조직 용해물을 위한 전체 단백질 샘플 보호 기능을 제공합니다.

Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X)의 특징:

• 완벽한 보호—all-in-one 칵테일은 단백질분해효소와 phosphatase 억제제를 함유
• 여러 패키지 크기—거의 모든 규모에 잘 맞는 편리한 4가지 패키지 크기 중에서 선택 가능
• 편의성 —100배 농축 액체 제형은 개별 억제제 및 다른 형식보다 사용하기에 훨씬 편리하고 효과적임
• 쉬운 보관—수성 형식이므로 DMSO 기반 칵테일과 달리 동결하지 않고 편리하게 저온 보관 가능
• 호환성—피크 전이를 유발할 수 있는 AEBSF를 함유하지 않기 때문에 질량분광분석법(MS)에서 사용 가능
• 검증 완료—Thermo Scientific Pierce Cell Lysis Buffer에서 완벽하게 사용 가능

Halt Protease and Phosphatase Inhibitor Cocktail은 특히 추출과 정제 중에 단백질이 분해되지 않도록 최적화되어 있습니다. 이 칵테일은 주요 등급의 단백질분해효소와 phosphatase에 대한 억제제를 함유하고 있어 aminopeptidase, cysteine 및 serine protease, serine/threonine 및 protein tyrosine phosphatase를 표적화합니다. metalloprotease 억제를 촉진할 수 있도록 별도의 튜브에 0.5M EDTA가 들어 있습니다.

관련 자료:
단백질분해효소 및 phosphatase 억제제 검토

관련 제품:
Protease and Phosphatase Inhibitor Cocktail 및 Tablet 전체
For Research Use Only. Not for use in diagnostic procedures.
사양
버퍼Lysis Buffer
설명Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-Free
억제제Protease Inhibitor, Phosphatase Inhibitor
수량24 x 100 μL
시약 유형Protease Inhibitor, Phosphatase Inhibitor
형태Liquid
제품라인Halt
제품 유형Protease and Phosphatase Inhibitor Cocktail
Unit SizeEach
구성 및 보관
Upon receipt store at 4°C

자주 묻는 질문(FAQ)

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Halt™ Protease and Phosphatase Inhibitor Single-Use Cocktail, EDTA-Free (100X), 24 x 100 μL FAQs

인용 및 참조 문헌 (5)

인용 및 참조 문헌
Abstract
Proteomic profiling across breast cancer cell lines and models.
Authors:Kalocsay M,Berberich MJ,Everley RA,Nariya MK,Chung M,Gaudio B,Victor C,Bradshaw GA,Eisert RJ,Hafner M,Sorger PK,Mills CE,Subramanian K
Journal:Scientific data
PubMed ID:37542042
We performed quantitative proteomics on 60 human-derived breast cancer cell line models to a depth of ~13,000 proteins. The resulting high-throughput datasets were assessed for quality and reproducibility. We used the datasets to identify and characterize the subtypes of breast cancer and showed that they conform to known transcriptional subtypes, ... More
Sting and p53 DNA repair pathways are compromised in Alzheimer's disease.
Authors:Nelson TJ,Xu Y
Journal:Scientific reports
PubMed ID:37221295
Alzheimer's disease (AD) is the most common cause of dementia. A common finding in AD is DNA damage. Double-strand DNA breaks (DSBs) are particularly hazardous to neurons because their post-mitotic state forces neurons to rely on error-prone and potentially mutagenic mechanisms to repair DNA breaks. However, it remains unclear whether ... More
FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity.
Authors:Bartley CM,O'Keefe RA,Bordey A
Journal:PloS one
PubMed ID:24806451
Hyperactive mammalian target of rapamycin (mTOR) is associated with cognitive deficits in several neurological disorders including tuberous sclerosis complex (TSC). The phosphorylation of the mRNA-binding protein FMRP reportedly depends on mTOR complex 1 (mTORC1) activity via p70 S6 kinase 1 (S6K1). Because this phosphorylation is thought to regulate the translation ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More
The Src inhibitor dasatinib accelerates the differentiation of human bone marrow-derived mesenchymal stromal cells into osteoblasts.
Authors:Id Boufker H, Lagneaux L, Najar M, Piccart M, Ghanem G, Body JJ, Journé F
Journal:BMC Cancer
PubMed ID:20565769
The proto-oncogene Src is an important non-receptor protein tyrosine kinase involved in signaling pathways that control cell adhesion, growth, migration and differentiation. It negatively regulates osteoblast activity, and, as such, its inhibition is a potential means to prevent bone loss. Dasatinib is a new dual Src/Bcr-Abl tyrosine kinase inhibitor initially ... More