Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
인용 및 참조 문헌 (6)
Thermo Scientific™

Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)

Thermo Scientific Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X)은 단일 용액의 편리함과 세포와 조직 용해물을 위한 전체 단백질 샘플자세히 알아보기
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카탈로그 번호수량
784455 x 1 mL
784411 mL
7844324 x 100 μL
7844710 mL
카탈로그 번호 78445
제품 가격(KRW)
1,080,000
Online offer
Ends: 31-Dec-2025
1,199,000
할인액 119,000 (10%)
Each
카트에 추가하기
수량:
5 x 1 mL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
1,080,000
Online offer
Ends: 31-Dec-2025
1,199,000
할인액 119,000 (10%)
Each
카트에 추가하기
Thermo Scientific Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X)은 단일 용액의 편리함과 세포와 조직 용해물을 위한 전체 단백질 샘플 보호 기능을 제공합니다.

Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X)의 특징:

• 완벽한 보호—all-in-one 칵테일은 단백질분해효소와 phosphatase 억제제를 함유
• 여러 패키지 크기—거의 모든 규모에 잘 맞는 편리한 4가지 패키지 크기 중에서 선택 가능
• 편의성 —100배 농축 액체 제형은 개별 억제제 및 다른 형식보다 사용하기에 훨씬 편리하고 효과적임
• 쉬운 보관—수성 형식이므로 DMSO 기반 칵테일과 달리 동결하지 않고 편리하게 저온 보관 가능
• 호환성—피크 전이를 유발할 수 있는 AEBSF를 함유하지 않기 때문에 질량분광분석법(MS)에서 사용 가능
• 검증 완료—Thermo Scientific Pierce Cell Lysis Buffer에서 완벽하게 사용 가능

Halt Protease and Phosphatase Inhibitor Cocktail은 특히 추출과 정제 중에 단백질이 분해되지 않도록 최적화되어 있습니다. 이 칵테일은 주요 등급의 단백질분해효소와 phosphatase에 대한 억제제를 함유하고 있어 aminopeptidase, cysteine 및 serine protease, serine/threonine 및 protein tyrosine phosphatase를 표적화합니다. metalloprotease 억제를 촉진할 수 있도록 별도의 튜브에 0.5M EDTA가 들어 있습니다.

관련 자료:
단백질분해효소 및 phosphatase 억제제 검토

관련 제품:
Protease and Phosphatase Inhibitor Cocktail 및 Tablet 전체
For Research Use Only. Not for use in diagnostic procedures.
사양
버퍼Lysis Buffer
설명Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-Free
억제제Protease Inhibitor, Phosphatase Inhibitor
수량5 x 1 mL
시약 유형Protease Inhibitor, Phosphatase Inhibitor
형태Liquid
제품라인Halt
제품 유형Protease and Phosphatase Inhibitor Cocktail
Unit SizeEach
구성 및 보관
Upon receipt store at 4°C. Product shipped with an ice pack. Do not freeze product.

자주 묻는 질문(FAQ)

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X), 5 x 1 mL FAQs

인용 및 참조 문헌 (6)

인용 및 참조 문헌
Abstract
Impact of carbamazepine on SMARCA4 (BRG1) expression in colorectal cancer: modulation by KRAS mutation status.
Authors:Shaykevich A,Chae D,Silverman I,Bassali J,Louloueian N,Siegman A,Bandyopadhyaya G,Goel S,Maitra R
Journal:Investigational new drugs
PubMed ID:38446332
SMARCA4 is a gene traditionally considered a tumor suppressor. Recent research has however found that SMARCA4 likely promotes cancer growth and is a good target for cancer treatment. The drug carbamazepine, an autophagy inducer, was used on colorectal cancer cell lines, HCT1116 and Hke3 (KRAS mutant and wildtype). Our study ... More
(3'R)-hydroxytabernaelegantine C: A bisindole alkaloid with potent apoptosis inducing activity in colon (HCT116, SW620) and liver (HepG2) cancer cells.
Authors:Paterna A,Gomes SE,Borralho PM,Mulhovo S,Rodrigues CM,Ferreira MU
Journal:Journal of ethnopharmacology
PubMed ID:27616029
ETHNOPHARMACOLOGICAL RELEVANCE: Tabernaemontana elegans Stapf. (Apocynaceae) is a medicinal plant traditionally used in African countries to treat cancer. AIMS OF THE STUDY: To discover new apoptosis inducing lead compounds from T. elegans and provide scientific validation of the ethnopharmacological use of this plant. MATERIALS AND METHODS: Through fractionation, (3'R)-hydroxytaberanelegantine C ... More
Authors:Mansoor TA, Borralho PM, Luo X, Mulhovo S, Rodrigues CM, Ferreira MJ.
Journal:J Nat Prod
PubMed ID:25066282
6-Acetonyldihydrochelerythrine (1), a benzophenanthridine alkaloid, isolated from the methanol extract of Zanthoxylum capense, displayed potent cytotoxic activity in human HCT116 and SW620 colon carcinoma cells, to a higher extent than 5-fluorouracil (5-FU), the cornerstone chemotherapeutic agent in colon cancer. Cytotoxicity of 1 was evaluated by MTS, lactate dehydrogenase (LDH), and ... More
CDK4/6 activity is required during G(2) arrest to prevent stress-induced endoreplication.
Authors:McKenney C,Lendner Y,Guerrero Zuniga A,Sinha N,Veresko B,Aikin TJ,Regot S
Journal:Science (New York, N.Y.)
PubMed ID:38696576
Cell cycle events are coordinated by cyclin-dependent kinases (CDKs) to ensure robust cell division. CDK4/6 and CDK2 regulate the growth 1 (G(1)) to synthesis (S) phase transition of the cell cycle by responding to mitogen signaling, promoting E2F transcription and inhibition of the anaphase-promoting complex. We found that this mechanism ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More
View all Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X), 5 x 1 mL citations