Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
인용 및 참조 문헌 (6)
Thermo Scientific™

Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)

Thermo Scientific Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X)은 단일 용액의 편리함과 세포와 조직 용해물을 위한 전체 단백질 샘플자세히 알아보기
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카탈로그 번호수량
7844710 mL
784411 mL
7844324 x 100 μL
784455 x 1 mL
카탈로그 번호 78447
제품 가격(KRW)
1,664,000
キャンペーン価格
Ends: 31-Dec-2025
1,848,000
할인액 184,000 (10%)
Each
카트에 추가하기
수량:
10 mL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
1,664,000
キャンペーン価格
Ends: 31-Dec-2025
1,848,000
할인액 184,000 (10%)
Each
카트에 추가하기
Thermo Scientific Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X)은 단일 용액의 편리함과 세포와 조직 용해물을 위한 전체 단백질 샘플 보호 기능을 제공합니다.

Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X)의 특징:

• 완벽한 보호—all-in-one 칵테일은 단백질분해효소와 phosphatase 억제제를 함유
• 여러 패키지 크기—거의 모든 규모에 잘 맞는 편리한 4가지 패키지 크기 중에서 선택 가능
• 편의성 —100배 농축 액체 제형은 개별 억제제 및 다른 형식보다 사용하기에 훨씬 편리하고 효과적임
• 쉬운 보관—수성 형식이므로 DMSO 기반 칵테일과 달리 동결하지 않고 편리하게 저온 보관 가능
• 호환성—피크 전이를 유발할 수 있는 AEBSF를 함유하지 않기 때문에 질량분광분석법(MS)에서 사용 가능
• 검증 완료—Thermo Scientific Pierce Cell Lysis Buffer에서 완벽하게 사용 가능

Halt Protease and Phosphatase Inhibitor Cocktail은 특히 추출과 정제 중에 단백질이 분해되지 않도록 최적화되어 있습니다. 이 칵테일은 주요 등급의 단백질분해효소와 phosphatase에 대한 억제제를 함유하고 있어 aminopeptidase, cysteine 및 serine protease, serine/threonine 및 protein tyrosine phosphatase를 표적화합니다. metalloprotease 억제를 촉진할 수 있도록 별도의 튜브에 0.5M EDTA가 들어 있습니다.

관련 자료:
단백질분해효소 및 phosphatase 억제제 검토

관련 제품:
Protease and Phosphatase Inhibitor Cocktail 및 Tablet 전체
For Research Use Only. Not for use in diagnostic procedures.
사양
버퍼Lysis Buffer
설명Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-Free
억제제Protease Inhibitor, Phosphatase Inhibitor
수량10 mL
시약 유형Protease Inhibitor, Phosphatase Inhibitor
형태Liquid
제품라인Halt
제품 유형Protease and Phosphatase Inhibitor Cocktail
Unit SizeEach
구성 및 보관
Upon receipt store at 4°C. Product shipped with an ice pack. Do not freeze product.

자주 묻는 질문(FAQ)

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X), 10 mL FAQs

인용 및 참조 문헌 (6)

인용 및 참조 문헌
Abstract
2-Aminopyridine-Based Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) Inhibitors: Assessment of Mechanism-Based Safety.
Authors:Dow RL,Ammirati M,Bagley SW,Bhattacharya SK,Buckbinder L,Cortes C,El-Kattan AF,Ford K,Freeman GB,Guimarães CRW,Liu S,Niosi M,Skoura A,Tess D
Journal:Journal of medicinal chemistry
PubMed ID:29570292
Studies have linked the serine-threonine kinase MAP4K4 to the regulation of a number of biological processes and/or diseases, including diabetes, cancer, inflammation, and angiogenesis. With a majority of the members of our lead series (e.g., 1) suffering from time-dependent inhibition (TDI) of CYP3A4, we sought design avenues that would eliminate ... More
Deficiency of endothelial sirtuin1 in mice stimulates skeletal muscle insulin sensitivity by modifying the secretome.
Authors:Li Q,Zhang Q,Kim YR,Gaddam RR,Jacobs JS,Bachschmid MM,Younis T,Zhu Z,Zingman L,London B,Rauckhorst AJ,Taylor EB,Norris AW,Vikram A,Irani K
Journal:Nature communications
PubMed ID:37696839
Downregulation of endothelial Sirtuin1 (Sirt1) in insulin resistant states contributes to vascular dysfunction. Furthermore, Sirt1 deficiency in skeletal myocytes promotes insulin resistance. Here, we show that deletion of endothelial Sirt1, while impairing endothelial function, paradoxically improves skeletal muscle insulin sensitivity. Compared to wild-type mice, male mice lacking endothelial Sirt1 (E-Sirt1-KO) ... More
Mito-Mendelian interactions alter in vivo glucose metabolism and insulin sensitivity in healthy mice.
Authors:Sammy MJ,Connelly AW,Brown JA,Holleman C,Habegger KM,Ballinger SW
Journal:American journal of physiology. Endocrinology and metabolism
PubMed ID:34370595
The regulation of euglycemia is essential for human health with both chronic hypoglycemia and hyperglycemia having detrimental effects. It is well documented that the incidence of type 2 diabetes increases with age and exhibits racial disparity. Interestingly, mitochondrial DNA (mtDNA) damage also accumulates with age and its sequence varies with ... More
Deciphering the interactome of Ataxin-2 and TDP-43 in iPSC-derived neurons for potential ALS targets.
Authors:Tian Y,Heinsinger N,Hu Y,Lim UM,Wang Y,Fernandis AZ,Parmentier-Batteur S,Klein B,Uslaner JM,Smith SM
Journal:PloS one
PubMed ID:39739690
Ataxin-2 is a protein containing a polyQ extension and intermediate length of polyQ extensions increases the risk of Amyotrophic Lateral Sclerosis (ALS). Down-regulation of Ataxin-2 has been shown to mitigate TDP-43 proteinopathy in ALS models. To identify alternative therapeutic targets that can mitigate TDP-43 toxicity, we examined the interaction between ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More
View all Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X), 10 mL citations