T-PER™ Tissue Protein Extraction Reagent
T-PER™ Tissue Protein Extraction Reagent
인용 및 참조 문헌 (15)
Thermo Scientific™

T-PER™ Tissue Protein Extraction Reagent

Thermo Scientific T-PER Tissue Protein Extraction Reagent는 조직 샘플에서 총 단백질을 약하게 추출할 수 있습니다.T-PER Tissue Protein Extraction Reagent의 특징:•자세히 알아보기
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카탈로그 번호수량
78510500 mL
카탈로그 번호 78510
제품 가격(KRW)
575,000
キャンペーン価格
Ends: 31-Mar-2026
638,000
할인액 63,000 (10%)
Each
카트에 추가하기
수량:
500 mL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
575,000
キャンペーン価格
Ends: 31-Mar-2026
638,000
할인액 63,000 (10%)
Each
카트에 추가하기
Thermo Scientific T-PER Tissue Protein Extraction Reagent는 조직 샘플에서 총 단백질을 약하게 추출할 수 있습니다.

T-PER Tissue Protein Extraction Reagent의 특징:

• 간단한 절차—조직과 T-PER Reagent 비율을 1:20(w/v)로 하고 조직 샘플을 균질화하고 나서 팰릿 세포나 조직 부산물을 원심분리함
• 가볍고 편리함—세척제를 투석하여 빠르고 쉽게 제거. 추가 성분(단백질분해효소 억제제, 염, 환원제 등)
• 융통성—리포터 분석, 단백질 키나아제 분석, 면역분석, 단백질 정제에서 용해물을 사용할 수 있음
• 호환성—용해물을 Pierce Coomassie Plus (Bradford) Protein Assay와 같은 표준 단백질 분석에서 사용할 수 있음

제품 세부 정보
이 조직 세포 용해 시약은 25 mM bicine, 150 mM sodium chloride (pH 7.6)에서 고유한 세척제를 사용해 균질화를 통해 포유류 조직 샘플에서 단백질 용약화의 효율성을 극대화합니다. 이 간단한 조성은 단백질분해효소 억제제, 염, 환원제, 킬레이트제와 함께 사용할 수 있어 매우 다양한 샘플 유형과 용해 어플리케이션에서 활용도가 높습니다. T-PER Reagent으로 조제된 세포 용해물은 리포터 분석(luciferase, beta-galactosidase, chloramphenicol acetyl transferase 등), 단백질 키나아제 분석(PKA, PKC, tyrosine kinase 등), 면역분석(웨스턴 블롯(Western Blot), ELISAs, RIAs), 단백질 정제 절차에서 직접 사용할 수 있습니다.

여러 샘플에서 균질하고 효율적인 조직 단백질을 추출하는 작업은 다양한 조직 조성과 추출 방법으로 인해 어렵습니다. 심장과 신장과 같은 섬유질 조직과 콜라겐 조직은 동결-해동을 통해서나 mortar 및 pestle 방법을 사용한 단백질 추출은 세척제를 사용하지 않으면 비효율적입니다. 반대로, 비장과 간과 같은 연조직은 보다 부드러운 추출 프로토콜을 사용해야만 최적의 결과를 얻을 수 있습니다. T-PER Reagent는 기계적 추출 방법에 상관 없이 모든 조직에서 사용하도록 고안되었습니다. 순한 세척제는 이 시약의 기능을 손상시키지 않으면서 단백질의 균질화를 강화해 주고, 중성 pH 및 염 농도는 세포 구획에서 보다 효율적인 추출을 가능하게 하여 균질한 용해물을 생성합니다.

T-PER Reagent은 냉동 조직 및 동결-해동 추출 프로토콜에 따라 새로운 제품과 냉동 제품 모두에서 단백질 수율이 검증되었습니다. 또한, T-PER Reagent은 뇌(synaptophysin)와 심장 조직(Troponin T Cardiac Isoform)의 조직 특이적 표지자로서 혈장막(Na-K+ ATPase, pan-cadherin), 세포질(HSP90, MAPK), 미토콘드리아(COXIV), 핵(HDAC1)과 같은 여러 세포 구획에서 표적 단백질을 효율적으로 추출하는 것으로 나타났습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
형식Liquid
수량500 mL
부피(미터법)500 mL
제품라인T-PER
제품 유형Tissue Protein Extraction Reagent
Unit SizeEach
구성 및 보관
Store at room temperature.

자주 묻는 질문(FAQ)

Which protein assays are the T-PER Tissue Protein Extraction Reagent (Cat. No. 78510) compatible with?

The T-PER Tissue Protein Extraction Reagent (Cat. No. 78510) is compatible with standard protein assays such as Pierce BCA Protein Assay (Cat. No. 23225) and Coomassie Plus (Bradford) Assay Kit (Cat. No. 23236).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the shelf life of T-PER Tissue Protein Extraction Reagent (Cat. No. 78510)?

T-PER Tissue Protein Extraction Reagent is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (room temperature). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can you provide the shelf-life for the T-PER Tissue Protein Extraction Reagent?

The T-PER Tissue Protein Extraction Reagent is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (room temperature). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

I only want to buy one reagent for extraction of protein from both mammalian cells and tissues. Will M-PER Mammalian Protein Extraction Reagent work for protein extraction from tissue samples?

For extraction of protein from tissue samples as well as some difficult-to-lyse mammalian cells, we recommend using T-PER Tissue Protein Extraction Reagent (Cat. No. 78510) with homogenization.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the basic extraction protocol using T-PER Tissue Protein Extraction Reagent?

Homogenize tissue sample in 1:20 (w/v) of tissue to T-PER Reagent, then centrifuge to pellet cell or tissue debris, and collect supernatant. For example, use a ratio of 100 mg of tissue to 2 mL T-PER Reagent. Use a smaller volume of reagent if a more concentrated protein extract is required.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all T-PER™ Tissue Protein Extraction Reagent FAQs

인용 및 참조 문헌 (15)

인용 및 참조 문헌
Abstract
Reverse phase protein microarray technology in traumatic brain injury.
Authors:Gyorgy AB,Walker J,Wingo D,Eidelman O,Pollard HB,Molnar A,Agoston DV
Journal:Journal of neuroscience methods
PubMed ID:20674607
Antibody based, high throughput proteomics technology represents an exciting new approach in understanding the pathobiologies of complex disorders such as cancer, stroke and traumatic brain injury. Reverse phase protein microarray (RPPA) can complement the classical methods based on mass spectrometry as a high throughput validation and quantification method. RPPA technology ... More
The concentration of brain homogenates with the Amicon Ultra Centrifugal filters.
Authors:Yang J,Sumbria RK
Journal:MethodsX
PubMed ID:35004217
Accurately measuring the brain concentration of a neurotherapeutic is critical in determining its pharmacokinetic profile in vivo. Biologics are potential therapeutics for neurologic diseases and biologics fused to an antibody targeting a transcytosis receptor at the Blood-Brain Barrier, designated as antibody-biologic fusion proteins, are Blood-Brain Barrier penetrating neurotherapeutics. The use ... More
Optimized Workflow for Proteomics and Phosphoproteomics With Limited Tissue Samples.
Authors:Hu M,Wang Y
Journal:Current protocols
PubMed ID:38646944
Proteomics and phosphoproteomics play crucial roles in elucidating the dynamics of post-transcriptional processes. While experimental methods and workflows have been established in this field, a persistent challenge arises when dealing with small samples containing a limited amount of protein. This limitation can significantly impact the recovery of peptides and phosphopeptides. ... More
MicroRNA-125b is a novel negative regulator of p53.
Authors:Le MT, Teh C, Shyh-Chang N, Xie H, Zhou B, Korzh V, Lodish HF, Lim B,
Journal:Genes Dev
PubMed ID:19293287
The p53 transcription factor is a key tumor suppressor and a central regulator of the stress response. To ensure a robust and precise response to cellular signals, p53 gene expression must be tightly regulated from the transcriptional to the post-translational levels. Computational predictions suggest that several microRNAs are involved in ... More
Apelin is necessary for the maintenance of insulin sensitivity.
Authors:Yue P, Jin H, Aillaud M, Deng AC, Azuma J, Asagami T, Kundu RK, Reaven GM, Quertermous T, Tsao PS
Journal:Am J Physiol Endocrinol Metab
PubMed ID:19861585
'The recently discovered peptide apelin is known to be involved in the maintenance of insulin sensitivity. However, questions persist regarding its precise role in the chronic setting. Fasting glucose, insulin, and adiponectin levels were determined on mice with generalized deficiency of apelin (APKO). Additionally, insulin (ITT) and glucose tolerance tests ... More
View all T-PER™ Tissue Protein Extraction Reagent citations