eBioscience™ BrdU Staining Kit for Flow Cytometry PerCP-eFluor™ 710
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Invitrogen™

eBioscience™ BrdU Staining Kit for Flow Cytometry PerCP-eFluor™ 710

이 BrdU Kit는 면역형광 검출을 이용한 면역조직화학(IHC)/면역세포화학(ICC) 분석에 사용되며, 면역조직화학 및 면역세포화학 분석을 통한 증식 세포의 식별 및 검사에 필요한자세히 알아보기
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카탈로그 번호수량
8846-6600-42100 tests
카탈로그 번호 8846-6600-42
제품 가격(KRW)
1,230,000
キャンペーン価格
Ends: 31-Mar-2026
1,447,000
할인액 217,000 (15%)
Each
카트에 추가하기
수량:
100 tests
제품 가격(KRW)
1,230,000
キャンペーン価格
Ends: 31-Mar-2026
1,447,000
할인액 217,000 (15%)
Each
카트에 추가하기
이 BrdU Kit는 면역형광 검출을 이용한 면역조직화학(IHC)/면역세포화학(ICC) 분석에 사용되며, 면역조직화학 및 면역세포화학 분석을 통한 증식 세포의 식별 및 검사에 필요한 시약과 완충액이 들어 있습니다. 세포 주기의 S-상 단계에서 티미딘 대신 DNA에 결합하는 티미딘 합성 아날로그인 5-브로모-2’-디옥시우리딘(BrdU)을 이용한 시험관 내 또는 체내 처리로 세포가 라벨링됩니다. 고정 및 항원 복구 단계에 이어 세포 또는 조직 절편이 BrdU 결합에 대해 염색되고 스트렙타비딘 결합 형광단을 이용하여 가시화됩니다. 이 키트는 냉동 및 포르말린 고정, 파라핀 포매 BrdU 라벨링 생쥐 장 조직을 이용한 IHC와 배양 슬라이드에서 증식된 BrdU 펄스 HeLa 세포 ICC 모두에 최적화되어 있습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Fluorescence
형식Kit
수량100 tests
용도(애플리케이션)BrdU Staining
용도 (장비)Flow Cytometer
제품라인eBioscience
제품 유형Staining Kit
Unit SizeEach

자주 묻는 질문(FAQ)

Can I also stain for intracellular targets such as transcription factors and cytokines using the eBioscience BrdU staining kit?

Yes, primary antibodies against additional intracellular targets can be added alongside the Anti-BrdU antibody. However, it is critical that you empirically determine whether these additional antibodies are compatible with this staining protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is the BrdU in the eBioscience BrdU staining kit supplied as a sterile formulation for in vivo use?

Yes, the BrdU is supplied as a sterile liquid. The vial should be opened under sterile conditions if it will be used in vivo.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I keep the diluted (1 mM) BrdU in the eBioscience BrdU staining kit at 4 degrees C? Can I refreeze it after use?

The stock (32.5 mM) BrdU can be frozen and thawed up to three times. Furthermore, the stock BrdU can be kept at 4 degrees C for up to one week. However, the diluted (1 mM) BrdU should be used immediately.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Have the BrdU Staining kits been tested on proliferating cells of different species?

We have used this kit to detect proliferating human and mouse cells. Other species have yet to be tested but feline, non-human primate and canine should also work.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the stopping points during flow cytometric analysis when using a BrdU staining kit?

Samples can be stored at 4 degrees C for up to 16 hours (i.e., overnight) after fixation with BrdU Staining Buffer (with or without washing in Flow Cytometry Staining Buffer) with no adverse effect on performance.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.