Pierce™ Protein A/G Magnetic Beads
Pierce™ Protein A/G Magnetic Beads
인용 및 참조 문헌 (11)
Thermo Scientific™

Pierce™ Protein A/G Magnetic Beads

Thermo Scientific Pierce Protein A/G Magnetic Bead는 수동식 또는 로봇 자기 분리기를 사용한 항체 정제와 면역침강법에 적합한 고품질 친화 입자입니다.Protein자세히 알아보기
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카탈로그 번호수량
888021 mL
888035 mL
카탈로그 번호 88802
제품 가격(KRW)
334,000
Online offer
Ends: 31-Dec-2025
371,000
할인액 37,000 (10%)
Each
카트에 추가하기
수량:
1 mL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
334,000
Online offer
Ends: 31-Dec-2025
371,000
할인액 37,000 (10%)
Each
카트에 추가하기
Thermo Scientific Pierce Protein A/G Magnetic Bead는 수동식 또는 로봇 자기 분리기를 사용한 항체 정제와 면역침강법에 적합한 고품질 친화 입자입니다.

Protein A/G Magnetic Bead의 특징:

• 고수용력—다른 제공업체의 일반 비드보다 거의 4배 높은 결합력 덕분에 실험별로 소량을 사용할 수 있음
• 비특이적 결합이 적음—사전 차단된 안정적인 비드는 깨끗한 정제 산물(예: 항체로 IP에서 용출된 항원은 복합체 IP 기질에서 단백질을 오염시키지 않음)을 생성함
• 융통성—하나의 비드에 Protein A와 Protein G의 IgG 결합 영역이 있는 편리함
• 호환성—비드를 수동 및 자동 어플리케이션(Thermo Scientific KingFisher Instrument 등)에서 사용할 수 있음
• 분석 일관성—마그네틱 비드는 수지 손실을 없애고 미세원심분리 튜브만을 사용하는 기존 IP 방법보다 효율적으로 용액을 분리함

이 마그네틱 비드는 Protein A와 Protein G의 IgG 결합 영역을 결합하는 유전적으로 가공된 재조합 융합 단백질인 Pierce Protein A/G로 코팅되어 있습니다. 따라서 단백질 단독보다는 다양한 종과 동형(isotype)에서 항체를 포획할 수 있습니다. 교차결합(crosslinker) 화학을 사용해 항체를 마그네틱 입자에 고정하여 면역침강된 샘플에서 IgG 오염을 방지할 수 있습니다. 이 비드는 마그네틱 스탠드에서 수동으로 사용할 수 있을 뿐 아니라 Thermo Scientific KingFisher Instrument과 같은 자동 플랫폼에서도 사용할 수 있습니다.

어플리케이션:
• IP 및 Co-IP 실험(전체 키트 참조)
• 환원되지 않은 조건에서 분석하기 위한 면역침강
• 항체 정제

Pierce Magnetic Bead에 고정된 재조합 Protein A/G는 Protein A와 Protein G의 IgG 결합 영역을 융합한 것입니다. Protein A/G에는 Protein A의 Fc-결합 영역 4개와 Protein G의 Fc-결합 영역 2개가 포함되어 있어 면역글로불린을 조사하고 정제할 때 편리한 도구로 사용할 수 있습니다. 또한, Pierce Magnetic Particle는 별도의 Protein A와 Protein G polypeptide의 혼합 고정이 아닌 것은 물론, Protein A 마그네틱 비드와 Protein G 마그네틱 비드의 혼합물도 아닙니다.

관련 자료:
단백질체학(proteomics)용 자동 마크네틱 분리 검토(선택 가이드 포함)

관련 제품:
Pierce Protein A/G Magnetic IP/Co-IP Kit
기타 Pierce Magnetic Bead
Pierce Protein A/G Agarose Bead

For Research Use Only. Not for use in diagnostic procedures.
사양
농도Slurry: 10mg/mL, 1% solids
리간드 유형Protein A/G
단백질 형태Recombinant
수량1 mL
타겟Antibody
용량(미터법)1 mL
형태Liquid
입자 크기1 μm
제품라인Pierce
유형Magnetic Affinity Bead
Unit SizeEach
구성 및 보관
Upon receipt store at 4°C. Product shipped with an ice pack.

자주 묻는 질문(FAQ)

Can I reuse Pierce Protein A/G Magnetic Beads (Cat. No. 88802, 88803)?

Pierce Protein A/G Magnetic Beads are intended for single use only. Therefore, we do not recommend reusing them.

What advantage do Pierce Magnetic Agarose beads have over Dynabeads or Pierce Magnetic Beads?

Pierce Magentic Agarose Beads have a much higher binding capacity.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I centrifuge the Thermo Scientific Protein A/G Magnetic Beads?

No, centrifuging causes the formation of irreversible aggregates which greatly reduces binding capacity.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How do I reduce non-specific binding to Thermo Scientific Protein A/G Magnetic Beads?

Adding 50-350 mM of NaCl to the Binding/Wash and Elution Buffers can help reduce non-specific bands. Also, use a low-pH elution for single-use applications. The optimal time for low-pH elution is 10 minutes; exceeding 10 minutes may result in non-specific binding and yield reduction.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

When should I avoid boiling the Thermo Scientific Protein A/G Magnetic Beads?

Avoid bead-boil elutions when using rabbit antibodies (primary or secondary) in downstream Western blot applications. Instead, perform elution in SDS-PAGE sample buffer at room temperature. For all other antibody species, boiling the beads in SDS-PAGE sample buffer is acceptable for single-use applications. Boiling could cause bead aggregation and loss of binding activity.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Pierce™ Protein A/G Magnetic Beads FAQs

인용 및 참조 문헌 (11)

인용 및 참조 문헌
Abstract
The Thyroid Hormone Analog GC-1 Mitigates Acute Lung Injury by Inhibiting M1 Macrophage Polarization.
Authors:Li B,Xia C,He W,Liu J,Duan R,Ji Z,Pan X,Zhou Y,Yu G,Wang L
Journal:Advanced science (Weinheim, Baden-Wurttemberg, Germany)
PubMed ID:39373388
Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a life-threatening condition with a high mortality rate of ≈40%. Thyroid hormones (THs) play crucial roles in maintaining homeostasis of the cellular microenvironment under stress. The previous studies confirmed that the clinical-stage TH analog GC-1 significantly alleviates pulmonary fibrosis by improving ... More
E3 ubiquitination ligase XIAP lightens diabetes-induced cognitive impairment by inactivating TXNIP-ERS-mediated neuronal injury.
Authors:Zhang Q,Huang HJ,Zhang JL,Tian Y,Hu Y
Journal:The Kaohsiung journal of medical sciences
PubMed ID:39629879
Diabetes-induced cognitive dysfunction (DCD) is a neurological disorder associated with diabetes, characterized by cognitive impairment driven by neuronal injury from chronic high glucose (HG) exposure. This study aims to elucidate the role and mechanisms of the X-linked inhibitor of apoptosis protein (XIAP)/thioredoxin-interacting protein (TXNIP) in hippocampal neuron cell death and ... More
Interplay Between Phosphorylation and O-GlcNAcylation of Sarcomeric Proteins in Ischemic Heart Failure.
Authors:Mercier T,Bouvet M,Dubois-Deruy E,Dechaumes A,Beseme O,Richard V,Mulder P,Pinet F
Journal:Frontiers in endocrinology
PubMed ID:30344511
Post-translational modifications (PTMs) of sarcomeric proteins could participate to left ventricular (LV) remodeling and contractile dysfunction leading in advanced heart failure (HF) with altered ejection fraction. Using an experimental rat model of HF (ligation of left coronary artery) and phosphoproteomic analysis, we identified an increase of desmin phosphorylation and a ... More
Extracellular matrix stiffness regulates degradation of MST2 via SCF (βTrCP).
Authors:Fiore APZP,Rodrigues AM,Ribeiro-Filho HV,Manucci AC,de Freitas Ribeiro P,Botelho MCS,Vogel C,Lopes-de-Oliveira PS,Pagano M,Bruni-Cardoso A
Journal:Biochimica et biophysica acta. General subjects
PubMed ID:36044955
The Hippo pathway plays central roles in relaying mechanical signals during development and tumorigenesis, but how the proteostasis of the Hippo kinase MST2 is regulated remains unknown. Here, we found that chemical inhibition of proteasomal proteolysis resulted in increased levels of MST2 in human breast epithelial cells. MST2 binds SCF(βTrCP) ... More
Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure.
Authors:Carreon TA,Castellanos A,Gasull X,Bhattacharya SK
Journal:Scientific reports
PubMed ID:28352076
In the eye, intraocular pressure (IOP) is tightly regulated and its persistent increase leads to ocular hypertension and glaucoma. We have previously shown that trabecular meshwork (TM) cells might detect aqueous humor fluid shear stress via interaction of the extracellular matrix (ECM) protein cochlin with the cell surface bound and ... More
View all Pierce™ Protein A/G Magnetic Beads citations