Pierce™ Glycoprotein Isolation Kit, WGA
Pierce™ Glycoprotein Isolation Kit, WGA
인용 및 참조 문헌 (3)
Thermo Scientific™

Pierce™ Glycoprotein Isolation Kit, WGA

The Thermo Scientific Pierce Glycoprotein Isolation Kit (Wheat Germ Agglutinin) allows isolation of glycoproteins from complex mixtures, including serum, tissue자세히 알아보기
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카탈로그 번호수량
8980510 reactions kit
카탈로그 번호 89805
제품 가격(KRW)
591,000
キャンペーン価格
Ends: 31-Dec-2025
695,000
할인액 104,000 (15%)
Each
카트에 추가하기
수량:
10 reactions kit
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
591,000
キャンペーン価格
Ends: 31-Dec-2025
695,000
할인액 104,000 (15%)
Each
카트에 추가하기
The Thermo Scientific Pierce Glycoprotein Isolation Kit (Wheat Germ Agglutinin) allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses wheat germ agglutinin (WGA) as a ligand to bind a specific class of glycoprotein from complex protein samples—those containing N-Acetyl glucosamine (GlcNAc) groups or sialic acid. This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

Features of this Glycoprotein Isolation Kit:

• High recovery—equivalent or greater glycoprotein recovery versus competitor kits and lectin resins
• Fast—glycoprotein purification in less than an hour
• Versatile—isolate glycoproteins from various sample types, e.g., human serum and cell lysate.
• Robust—lectin does not leach from resin when processing sample
• Convenient—complete kit offering lectin resins and spin columns with all necessary reagents
• Compatible with Bradford-based protein assays—dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay

Related Products
Pierce™ Glycoprotein Isolation Kit, ConA
For Research Use Only. Not for use in diagnostic procedures.
사양
컬럼 유형Affinity, Agarose Resin
설명Pierce Glycoprotein Isolation Kit, WGA
형식Kit, Microcentrifuge, Spin Column
수량10 reactions kit
정지상Lectin
충분10 Reactions
타겟Glycoprotein
제품라인Pierce
유형Glycoprotein Isolation Kit
Unit SizeEach
구성 및 보관
Sufficient For: Isolating glycoproteins with strong affinity for WGA from 10 samples of up to 640 μL (1 to 1.5 mg total protein) each
• WGA Lectin Resin, 1.1 mL
• Binding/Wash Buffer (5X), 6.5 mL
• Elution Buffer, 5 mL
• Spin Columns, 10 columns
• Collection Tubes, 20 tubes

Store at 4°C.

자주 묻는 질문(FAQ)

What is the difference between your Pierce Glycoprotein Isolation Kit, ConA and WGA?

The ConA kit (Cat. No. 89804), which has concanavalin A immobilized on the resin, recognizes alpha-linked mannose, and to a lesser extent, terminal glucose residues. The WGA kit (Cat. No. 89805), which has wheat germ agglutinin immobilized on the resin, recognizes N-acetyl glucosamine (GlcNAc) groups and sialic acid.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I isolate my proteins based on their post-translational modification (PTM) or active binding site?

Enrichment of specific proteins or protein complexes can most easily be accomplished by using target-specific immobilized antibody or PTM specific affinity binding. We offer several kits targeting phosphorylated proteins, alpha- linked mannose and terminal glucose residues, N-acetyl glucosamine, polyubiquinated proteins, ATP binding sites, and active serine hydrolase enzymes. Choose the right protein enrichment kit for your application using the following link (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-purification/protein-enrichment.html).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

인용 및 참조 문헌 (3)

인용 및 참조 문헌
Abstract
Lectin affinity as an approach to the proteomic analysis of membrane glycoproteins.
Authors:Ghosh D, Krokhin O, Antonovici M, Ens W, Standing KG, Beavis RC, Wilkins JA
Journal:J Proteome Res
PubMed ID:15359739
The aim was to determine the proportion of membrane glycoproteins captured using concanavalin A or wheat germ agglutinin lectin affinity chromatography. Digests of the isolated proteins were separated by reversed-phase liquid chromatography and analyzed by matrix-assisted laser desorption tandem mass spectrometry. The two lectins identified different groups of proteins with ... More
Identification of Potential Glycoprotein Biomarkers in Estrogen Receptor Positive (ER+) and Negative (ER-) Human Breast Cancer Tissues by LC-LTQ/FT-ICR Mass Spectrometry.
Authors:Semaan SM, Wang X, Marshall AG, Sang QX
Journal:J Cancer
PubMed ID:22773931
Breast cancer is the second most fatal cancer in American women. To increase the life expectancy of patients with breast cancer new diagnostic and prognostic biomarkers and drug targets must be identified. A change in the glycosylation on a glycoprotein often causes a change in the function of that glycoprotein; ... More
GlycoSuiteDB: a new curated relational database of glycoprotein glycan structures and their biological sources.
Authors:Cooper CA, Harrison MJ, Wilkins MR, Packer NH
Journal:Nucleic Acids Res
PubMed ID:11125129
GlycoSuiteDB is a relational database that curates information from the scientific literature on glyco-protein derived glycan structures, their biological sources, the references in which the glycan was described and the methods used to determine the glycan structure. To date, the database includes most published O:-linked oligosaccharides from the last 50 ... More