Pierce™ Centrifuge Columns

Pierce Centrifuge Columns

Thermo Scientific Pierce centrifuge columns are convenient tools for handling 40 μL to 20 mL of an affinity support for purification. The columns are made from low-protein-binding polypropylene and contain a polyethylene filter (~30-μm pore size) for compatibility with protein purification.

Simply add the affinity resin to the polypropylene column, remove the twist-off bottom, and allow the resin to pack itself. Then add your sample, allow it to bind to the support, and use a centrifuge to efficiently wash away any contaminants and elute your purified protein.

Pierce centrifuge columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and binding steps with dilute samples when slow processing time is desired, column extenders can be used to increase the column reservoir capacity. Use any combination of processing techniques to get the best possible chromatography results.

0.8 mL columns

• Maximum volume: 800 μL
• Recommended resin volume: 40–400 μL
• Receiver tube: fits standard microcentrifuge tubes
• Cap: O-ring screw-top
• Twist-off bottom

2 mL columns

• Maximum volume: 5 mL (2 mL resin bed, 3 mL sample)
• Recommended resin volume: 400 μL–2 mL
• Receiver tube: fits standard 15 mL conical centrifuge tubes
• Cap: screw-top
• Twist-off bottom and press-on cap to reseal

5 mL columns

• Maximum volume: 8 mL (5 mL resin bed, 3 mL sample)
• Recommended resin volume: 1–5 mL
• Receiver tube: fits standard 15 mL conical centrifuge tubes
• Cap: screw-top
• Twist-off bottom and press-on cap to reseal

10 mL columns

• Maximum volume: 22 mL (10 mL resin bed, 12 mL sample)
• Recommended resin volume: 2–10 mL
• Receiver tube: fits standard 50 mL conical centrifuge tubes
• Cap: screw-top
• Twist-off bottom and press-on cap to reseal

20 mL columns

• Maximum volume: 34 mL (20 mL resin bed, 14 mL sample)
• Recommended resin volume: 4–20 mL
• Receiver tube: fits standard 50 mL conical centrifuge tubes
• Cap: screw-top
• Twist-off bottom and press-on cap to reseal

Applications

• Affinity purification, affinity chromatography
• Immuno-depletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange