Pierce™ Strong Cation Exchange and Strong Anion Exchange Spin Columns
Pierce™ Strong Cation Exchange and Strong Anion Exchange Spin Columns
Thermo Scientific™

Pierce™ Strong Cation Exchange and Strong Anion Exchange Spin Columns

Perform rapid protein fractionation and sample preparation based on positive and negative charge differences using Pierce Strong Cation and Anion Exchange Spin Columns, in mini (4 mg) and maxi (80 mg) binding formats.
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호설명
90011Pierce™ Strong Anion Exchange Spin Columns, Maxi
90009Pierce™ Strong Cation Exchange Spin Columns, Maxi
90008Pierce™ Strong Cation Exchange Spin Columns, Mini
90010Pierce™ Strong Anion Exchange Spin Columns, Mini
4 옵션
카탈로그 번호 90011
제품 정가(KRW)/ 8 columns
제품 정가502,000
온라인 행사452,000온라인 주문시에만 적용 (Supply Center 제외) .
(ends 01-Jan-2025)
공급가
-
카트에 추가하기
설명:
Pierce™ Strong Anion Exchange Spin Columns, Maxi
제품 정가(KRW)/ 8 columns
온라인 행사452,000온라인 주문시에만 적용 (Supply Center 제외) .
(ends 01-Jan-2025)
카트에 추가하기
Pierce™ Strong Cation Exchange and Strong Anion Exchange Spin Columns
카탈로그 번호90011
제품 정가(KRW)/ 8 columns
온라인 행사452,000온라인 주문시에만 적용 (Supply Center 제외) .
(ends 01-Jan-2025)
-
카트에 추가하기
Realize shorter diffusion times, higher protein recovery, and intact biological activity of eluted samples with Pierce Strong Cation Exchange and Strong Anion Exchange spin columns. Unlike resin-based column chromatography, these columns utilize ion exchange chromatography to separate molecules based on differences in their accessible surface charges.
Pierce Strong Cation Exchange and Strong Anion Exchange spin columns use membrane-adsorption as the chromatographic method of choice to fractionate proteins based on their charge differences. The column matrix has a highly porous structure, with pores larger than 3000 nm, giving proteins ready accessibility to the membrane's charged ligands. These adsorptive membranes maintain high efficiencies at high flow rates and during fractionation of large biomolecules with small diffusivities.

Ion chromatography is widely preferred in the pre-fractionation or purification of a target protein from crude biological samples. Interactions between molecules and active sites on the ion exchange membrane occur in a convective manner via pores, which shortens diffusion time when compared with fluid inside the pores of a resin particle. Also, the relatively mild binding and eluting conditions of this separation method produce high protein recovery with intact biological activity.Pierce strong ion exchange spin columns enable rapid protein fractionation and sample preparation based on charge differences. There are two available formats:
Mini Format: for binding up to 4 mg (500 μL capacity)
Maxi Format: for binding up to 80 mg (20 mL capacity)

Features of Pierce strong ion exchange spin columns:
Fast and simple—membrane-based spin format eliminates column packing
Convenient and expandable—centrifugal format enables convenient processing of multiple samples in parallel
Robust—membrane adsorber spin columns do not crack or run dry
Low bed volume—small membrane adsorber bed volumes make working with low buffer volumes possible, leading to concentrated elution fractions

Strong ion exchange spin column applications
• Pre-fractionation of proteins in lysate
• Scouting purification conditions for new protein preparation protocols
• Removal of endotoxins from monoclonal antibodies
• Polishing His-tagged proteins after metal chelate chromatography
• Purification and concentration of proteins
• Purification of antibodies from serum, ascites, or tissue culture supernatant
• Removal of detergent from protein solutions
• Sample preparation before 1D or 2D PAGE
• Purification of phosphopeptides before MS analysis

For Research Use Only. Not for use in diagnostic procedures.
사양
설명Pierce™ Strong Anion Exchange Spin Columns, Maxi
결합 강도Strong
형식Spin Column
컬럼 유형Cellulose Membrane, Ion Exchange
정지상Charged Ligand
수량8 Columns
제품라인Pierce™
샘플 부피80 mg, 20 mL
Unit Size8 columns
구성 및 보관
Upon receipt store at room temperature.