GeneChip™ miRNA 3.1 Array Plate

The hybridization mix for PEG arrays is different from the hybridization mix for cartridges. The concentrations of the hybridization mix are different and in addition, DMSO is not added into the hybridization mix for PEG arrays because it can affect the glue that holds the PEG to the plate.

The species information can be found in the annotation file of the miRNA array of interest.
Please complete the following steps:
- Go to www.thermofisher.com and go to the product page of the array of interest.
- Scroll down to support files.
- Download the annotation files for the miRNA array of interest, and open in excel.
- In the file, there will be a column, species scientific name. This column will have all the species present on the array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Due to certain miRNAs being conserved across species, there may be multiple copies of the same probe across the miRNA arrays. However, sample processing with the FlashTag Biotin HSR RNA Labeling Kits ensures that there is excess target compared to the probes on the array. Consequently, there is enough sample available to hybridize onto all the replicate probes.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.