Is the Clariom S capable of exon-level analysis?
No, the Clariom S array is not capable of exon-level analysis. The probes have selected to be the most constitutive probes, representing the most common areas of a gene model. This means that most of the exons in the gene model will likely not have representation on the array, meaning that it is not practical to enable exon-level analysis.
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What are the Clariom Assay scan times and file sizes?
Clariom D Assay - scan time is about 33 min.
- DAT file size about 790-800MB
- CEL file size about 68 MB
The Clariom S - scan time is about 5 min.
- DAT file size about 35-40MB
- CEL file size about 3 MB
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Is globin reduction of blood samples required when using the Clariom S assays?
No, globin reduction is not necessary for the Clariom S assay. The Clariom S assay is designed to preserve sample integrity and reduce data variability without requiring a globin or rRNA removal step. This means that you can perform the Clariom S assay without the need to perform globin reduction on your samples.
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What is the correct fluidics script for the Clariom S (human, mouse and rat) array?
The Clariom S array is a 400 format, the correct script is FS450_0007.
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How do I install Expression Console Software and TAC Software library files for human, mouse, or rat transcriptome assays?
Analysis library files and annotation files can be downloaded from within the TAC Software. Please refer to the respective user manuals for detailed instructions.
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