Pierce™ Antibody Biotinylation Kit for IP

The Thermo Scientific™ Pierce™ Antibody Biotinylation Kit for IP provides biotinylation reagents designed specifically for the labeling of primary antibodies used in immunoprecipitation applications.

• Optimized for IP - reagents and protocols developed specifically to address antibody labeling for immunoprecipitation applications
• Easy to use - kit configuration specifically optimized to accommodate 50 to 200μg of antibody in a 100μL reaction; desalting columns are provided to remove excess biotinylation reagent after labeling
• Amine reactive - reacts with primary amines (-NH2) of antibodies
• Enhanced solubility - pegylation improves water solubility of the biotinylated antibody and prevents aggregation upon storage
• Reduced steric hindrance - longer spacer arm (29 angstroms) minimize steric hindrance when binding to avidin molecules

The kit contains sufficient reagents to label 50 to 200μg of antibody in 100μL reaction volumes for eight samples. The NHS-PEG4-biotin labeling reagent contains an amine-reactive N-hydroxysuccinimide ester (NHS) group and a water-soluble PEG4 spacer for optimal labeling and is provided in easy-to-use, single-use microtubes. Both the labeling efficiency of the biotinylation reagent and binding affinity of the labeled antibody have been validated using mouse monoclonal (IgG1, IgG2), rabbit polyconal, and rabbit monoclonal antibodies. Zeba™ Desalting Spin Columns are provided for easy and efficient removal of salts and excess biotin.

Applications:

• IP and co-IP
• IP and co-IP for mass spectrometry applications
• Antibody attachment to streptavidin agarose and magnetic supports