CHO DG44 Cells (cGMP banked) and Media Kit

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Gibco™

CHO DG44 Cells (cGMP banked) and Media Kit

CHO DG44 Cells (cGMP banked) & Media Kit는 현탁 배양에서 DHFR- CHO(dihydrofolate reductase deficient Chinese Hamster Ovary) 세포 성장과 재조합자세히 알아보기

카탈로그 번호	수량
A1100001	1 kit

카탈로그 번호 A1100001

제품 가격(KRW)

1 kit

CHO DG44 Cells (cGMP banked) & Media Kit는 현탁 배양에서 DHFR- CHO(dihydrofolate reductase deficient Chinese Hamster Ovary) 세포 성장과 재조합 단백질 발현을 위해 개발되었습니다. kit 제공:

GIBCO™ CD DG44 Medium—a 화학적으로 정의된 무단백 배지로 현택 배양에서 DG44 세포의 성장과 발현을 최적화합니다.

CHO DG44 cells (cGMP banked) —Parental CHO DG44 cell은 cGMP 품질 표준에 부합하게 생산, 보관, 검사되었습니다. 이들은 CD DG44 Medium에 맞추어져 있으며 세포 성장과 transfection efficiency가 우수합니다.

또한 CHO DG44 Cells (cGMP banked) & Media Kit에는 화학적으로 정의된 무단백 환경에서 DG44 세포의 성장과 단백질 발현을 최적화하기 위해 L-glutamine와 Pluronic™ F-68이 들어있습니다.

For Research Use Only. Not for use in diagnostic procedures.

분류Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free

미디어 권장 사항CD DG44 Medium

셀 수1 x 10⁷

제품라인Gibco

수량1 kit

세포주CHO DG44

Culture TypeSuspension Cell Culture

종Hamster

Unit SizeEach

구성 및 보관

CHO DG44 Cells (cGMP banked) and Media Kit (SKU A1100001) contains:

• CD DG44 Medium 1000 mL- 12610-010, store in the dark at 2-8°C
• CHO DG44 Cells (cGMP banked) 1 vial (> 1x10^7 cells) - A1097101 (see limited label license), store in liquid nitrogen
• PLURONIC F-68, 10% (100X) 100 mL - 24040-032, store at room temperature
• L-glutamine-200mM (100X) 100 mL - 25030-081 or 25030-024 (CE marked version for Europe only), store at -20°C

자주 묻는 질문(FAQ)

Has the DG44 cell line been used for the large scale production of any FDA-approved biotherapeutic proteins?

70% of all therapeutics produced in mammalian cells are expressed in CHO cells. CHO cells are preferred because of human-like glycosylation and no propagation of human-like viruses. Most therapeutic IgGs are produced using the CHO platform and a number of FDA-approved biotherapeutics have been produced in DG44 cells.

Why are the defrosted CHO DG44 Cells (cGMP banked) transferred directly into pre-warmed media instead of being centrifuged for recovery when using the CHO DG44 Cells (cGMP banked) and Media Kit (Cat. No. A1100001)?

The defrosted contents of the vial of Gibco CHO DG44 Cells are transferred directly to 29 mL of pre-warmed media and then incubated because the cells are extremely fragile upon recovery from cryopreservation. Centrifuging the cells at this stage could cause significant damage, reducing their viability and recovery efficiency. To help ensure optimal recovery and viability, the cells are directly transferred to pre-warmed media and incubated under optimal conditions. This approach is also why we do not mention centrifuge resuspension in the subculture section. We have found that the shear forces during centrifugation are more harmful to the cells than retaining a small amount of old media or DMSO. The volume of media in the flask has been found to sufficiently dilute the DMSO such that it does not impact recovery or cell growth rather than using a centrifuge.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I learn more about commercial licensing of Freedom kits, ExpiCHO-S Cells (cGMP banked), CHO-S Cells (cGMP banked), and CHO DG44 Cells (cGMP banked)?

To inquire about a Commercial Production or Service License, please email: outlicensing@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the term of the license agreement for ExpiCHO-S Cells (cGMP banked), CHO-S Cells (cGMP banked), and CHO DG44 Cells (cGMP banked)?

Licensing rights are granted in perpetuity for the Commercial Production License. The term of the Commercial Service License is negotiated between the parties.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Is there a pull-through royalty on final products or an annual maintenance fee for using ExpiCHO-S Cells (cGMP banked), CHO-S Cells (cGMP banked), and CHO DG44 Cells (cGMP banked)??

Only a one-time license fee is required for a Commercial Production License, and it covers use during the clinical trial process and commercialization of an unlimited number of molecules.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

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