Blasticidin S HCl (10 mg/mL)
Blasticidin S HCl (10 mg/mL)
인용 및 참조 문헌 (5)
Gibco™

Blasticidin S HCl (10 mg/mL)

Blasticidin S는 Streptomyces griseochromogenes에서 분리한 뉴클레오시드 항생제입니다. 이는 원핵세포와 진핵세포 모두에서 단백질 합성을 억제합니다. Blasticidin S는 ribosome으로 펩티드 결합 형성을자세히 알아보기
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카탈로그 번호수량
A111390220 mL
A111390310 x 1 mL
카탈로그 번호 A1113902
제품 가격(KRW)
1,573,000
Online offer
Ends: 31-Dec-2025
1,655,000
할인액 82,000 (5%)
Each
카트에 추가하기
수량:
20 mL
제품 가격(KRW)
1,573,000
Online offer
Ends: 31-Dec-2025
1,655,000
할인액 82,000 (5%)
Each
카트에 추가하기
Blasticidin S는 Streptomyces griseochromogenes에서 분리한 뉴클레오시드 항생제입니다. 이는 원핵세포와 진핵세포 모두에서 단백질 합성을 억제합니다. Blasticidin S는 ribosome으로 펩티드 결합 형성을 억제하여 신속하게 작용합니다.. Blasticidin S는 세포 배양의 오염을 방지하기 위해 사용합니다. 권장 작업 농도는 cell line에 따라 2∼ 20 μg ⁄ ml 범위입니다. 이 제품은 산제로 제공되며 10 mg/ml 농도 20mM HEPES (pH 7.2-7.5)를 사용해 stock solution으로 만들어야 합니다. 라이프 테크놀로지스는 세포 배양 어플리케이션에 사용하는 다양한 항생∙항진균제 를 제공합니다.

또한 Blasticidin S는 50 - 100 μg ⁄ ml 농도에서 박테리아 선택적인 항생제로 사용됩니다. 내성은 Blasticidin S deaminase 유전자 BSD (2) 또는 bsr 중 하나의 발현으로 확인합니다.이 deaminases가 Blasticidin S를 무독성 변이체로 바꿉니다. 선택 용으로는 LB medium의 염함유량은 낮고(<90 mM) pH는 7.0를 초과하지 않아야 Basticidin S 활성을 유지할 수 있습니다. 라이프테크놀로지스는 다양한선택 항생제를 제공합니다.

용도

이 제품은 연구용으로만 사용해야 합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Eukaryotic Cells, Prokaryotic Cells
농도10 mg/mL
배양 유형Mammalian Cell Culture, Insect Cell Culture
제품라인Gibco
수량20 mL
유통 기한9 Months
형태Liquid
제품 유형Antibiotic
멸균Sterile-filtered
첨가제 포함HEPES
Unit SizeEach
구성 및 보관
Storage conditions: -5°C to -20°C (protect from light)
Shipping conditions: Dry ice
Shelf life: 9 months from date of manufacture

자주 묻는 질문(FAQ)

Which of your antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for stable selection in mammalian cells?

All of our antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for making multiple stable cell lines. However, kill curves will need to be performed for each combination of antibiotics since sensitivity to a given antibiotic tends to increase when combined with other antibiotics.

What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

What is the mode of action on the following antibiotics: Blasticidin, Geneticin (G418), Hygromycin, and Zeocin?

Blasticidin: Nucleoside Inhibits protein synthesis in prokaryotic and eukaryotic cells by interfering with peptidyl transfer reaction of protein synthesis, causing early termination of translation.

Geneticin (G418): Aminoglycoside Blocks protein synthesis in mammalian cells by interfering with ribosomal function.

Hygromycin: Aminocyclitol Inhibits protein synthesis by disrupting translocation and promoting mistranslation.

Zeocin: Intercalates with DNA and cleaves it.

What is the optimal pH of low salt LB for LB + blasticidin plates?

We recommend a pH of 7 or less and half the normal amount of NaCl in your LB media or plates.

See the following paper for details on optimal conditions: Yamaguchi et al (1965) Inhibition of Protein Synthesis by Blasticidin S. Journal of Biochemistry (Tokyo) Volume 57: pp 667-677.

How long can Blasticidin be stored at 4 degrees C after thawing? Does the unused portion have to be discarded after thawing?

Blasticidin is stable for 6 months when stored at 4 degrees C. Discard remaining material after this time.

View all Blasticidin S HCl (10 mg/mL), 20 mL FAQs

인용 및 참조 문헌 (5)

인용 및 참조 문헌
Abstract
A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing.
Authors:Jones EM, Jajoo R, Cancilla D, Lubock NB, Wang J, Satyadi M, Cheung R, de March C, Bloom JS, Matsunami H, Kosuri S
Journal:Cell Syst
PubMed ID:30904378
'G protein-coupled receptors (GPCRs) are central to how mammalian cells sense and respond to chemicals. Mammalian olfactory receptors (ORs), the largest family of GPCRs, mediate the sense of smell through activation by small molecules, though for most bonafide ligands, they have not been identified. Here, we introduce a platform to ... More
A Brain Penetrant Mutant IDH1 Inhibitor Provides In Vivo Survival Benefit.
Authors:Kopinja J, Sevilla RS, Levitan D, Dai D, Vanko A, Spooner E, Ware C, Forget R, Hu K, Kral A, Spacciapoli P, Kennan R, Jayaraman L, Pucci V, Perera S, Zhang W, Fischer C, Lam MH
Journal:Sci Rep
PubMed ID:29062039
'Mutations in IDH1 are highly prevalent in human glioma. First line treatment is radiotherapy, which many patients often forego to avoid treatment-associated morbidities. The high prevalence of IDH1 mutations in glioma highlights the need for brain-penetrant IDH1 mutant-selective inhibitors as an alternative therapeutic option. Here, we have explored the utility ... More
Biological Characterization of a Stable Effector Functionless (SEFL) Monoclonal Antibody Scaffold in Vitro.
Authors:Liu L, Jacobsen FW, Everds N, Zhuang Y, Yu YB, Li N, Clark D, Nguyen MP, Fort M, Narayanan P, Kim K, Stevenson R, Narhi L, Gunasekaran K, Bussiere JL
Journal:J Biol Chem
PubMed ID:27994063
The stable effector functionLess (SEFL) antibody was designed as an IgG1 antibody with a constant region that lacks the ability to interact with Fc? receptors. The engineering and stability and pharmacokinetic assessments of the SEFL scaffold is described in the accompanying article (Jacobsen, F. W., Stevenson, R., Li, C., Salimi-Moosavi, ... More
Proteomics reveals Rictor as a noncanonical TGF-ß signaling target during aneurysm progression in Marfan mice.
Authors:Parker SJ, Stotland A, MacFarlane E, Wilson N, Orosco A, Venkatraman V, Madrid K, Gottlieb R, Dietz HC, Van Eyk JE
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:30004239
The objective of the present study was to 1) analyze the ascending aortic proteome within a mouse model of Marfan syndrome (MFS; Fbn1
Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair.
Authors:Nakazawa Y, Hara Y, Oka Y, Komine O, van den Heuvel D, Guo C, Daigaku Y, Isono M, He Y, Shimada M, Kato K, Jia N, Hashimoto S, Kotani Y, Miyoshi Y, Tanaka M, Sobue A, Mitsutake N, Suganami T, Masuda A, Ohno K, Nakada S, Mashimo T, Yamanaka K, Luijsterburg MS, Ogi T
Journal:Cell
PubMed ID:32142649
Transcription-coupled nucleotide excision repair (TC-NER) is initiated by the stalling of elongating RNA polymerase II (RNAPIIo) at DNA lesions. The ubiquitination of RNAPIIo in response to DNA damage is an evolutionarily conserved event, but its function in mammals is unknown. Here, we identified a single DNA damage-induced ubiquitination site in ... More