Blasticidin S HCl (10 mg/mL)
Blasticidin S HCl (10 mg/mL)
인용 및 참조 문헌 (7)
Gibco™

Blasticidin S HCl (10 mg/mL)

Blasticidin S는 Streptomyces griseochromogenes에서 분리한 뉴클레오시드 항생제입니다. 이는 원핵세포와 진핵세포 모두에서 단백질 합성을 억제합니다. Blasticidin S는 ribosome으로 펩티드 결합 형성을자세히 알아보기
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카탈로그 번호수량
A111390310 x 1 mL
A111390220 mL
카탈로그 번호 A1113903
제품 가격(KRW)
778,000
Online offer
Ends: 31-Dec-2025
818,000
할인액 40,000 (5%)
Each
카트에 추가하기
수량:
10 x 1 mL
제품 가격(KRW)
778,000
Online offer
Ends: 31-Dec-2025
818,000
할인액 40,000 (5%)
Each
카트에 추가하기
Blasticidin S는 Streptomyces griseochromogenes에서 분리한 뉴클레오시드 항생제입니다. 이는 원핵세포와 진핵세포 모두에서 단백질 합성을 억제합니다. Blasticidin S는 ribosome으로 펩티드 결합 형성을 억제하여 신속하게 작용합니다.. Blasticidin S는 세포 배양의 오염을 방지하기 위해 사용합니다. 권장 작업 농도는 cell line에 따라 2∼ 20 μg ⁄ ml 범위입니다. 이 제품은 산제로 제공되며 10 mg/ml 농도 20mM HEPES (pH 7.2-7.5)를 사용해 stock solution으로 만들어야 합니다. 라이프 테크놀로지스는 세포 배양 어플리케이션에 사용하는 다양한 항생∙항진균제 를 제공합니다.

또한 Blasticidin S는 50 - 100 μg ⁄ ml 농도에서 박테리아 선택적인 항생제로 사용됩니다. 내성은 Blasticidin S deaminase 유전자 BSD (2) 또는 bsr 중 하나의 발현으로 확인합니다.이 deaminases가 Blasticidin S를 무독성 변이체로 바꿉니다. 선택 용으로는 LB medium의 염함유량은 낮고(<90 mM) pH는 7.0를 초과하지 않아야 Basticidin S 활성을 유지할 수 있습니다. 라이프테크놀로지스는 다양한선택 항생제를 제공합니다.

용도

이 제품은 연구용으로만 사용해야 합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Eukaryotic Cells, Prokaryotic Cells
농도10 mg/mL
배양 유형Mammalian Cell Culture, Insect Cell Culture
제품라인Gibco
수량10 x 1 mL
유통 기한9 Months
형태Liquid
제품 유형Antibiotic
멸균Sterile-filtered
첨가제 포함HEPES
Unit SizeEach
구성 및 보관
Storage conditions: -5°C to -20°C (protect from light)
Shipping conditions: Dry ice
Shelf life: 9 months from date of manufacture

자주 묻는 질문(FAQ)

Which of your antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for stable selection in mammalian cells?

All of our antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for making multiple stable cell lines. However, kill curves will need to be performed for each combination of antibiotics since sensitivity to a given antibiotic tends to increase when combined with other antibiotics.

What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

What is the mode of action on the following antibiotics: Blasticidin, Geneticin (G418), Hygromycin, and Zeocin?

Blasticidin: Nucleoside Inhibits protein synthesis in prokaryotic and eukaryotic cells by interfering with peptidyl transfer reaction of protein synthesis, causing early termination of translation.

Geneticin (G418): Aminoglycoside Blocks protein synthesis in mammalian cells by interfering with ribosomal function.

Hygromycin: Aminocyclitol Inhibits protein synthesis by disrupting translocation and promoting mistranslation.

Zeocin: Intercalates with DNA and cleaves it.

What is the optimal pH of low salt LB for LB + blasticidin plates?

We recommend a pH of 7 or less and half the normal amount of NaCl in your LB media or plates.

See the following paper for details on optimal conditions: Yamaguchi et al (1965) Inhibition of Protein Synthesis by Blasticidin S. Journal of Biochemistry (Tokyo) Volume 57: pp 667-677.

How long can Blasticidin be stored at 4 degrees C after thawing? Does the unused portion have to be discarded after thawing?

Blasticidin is stable for 6 months when stored at 4 degrees C. Discard remaining material after this time.

View all Blasticidin S HCl (10 mg/mL), 10 x 1 mL FAQs

인용 및 참조 문헌 (7)

인용 및 참조 문헌
Abstract
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Authors:Behan FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ
Journal:Nature
PubMed ID:30971826
'Functional genomics approaches can overcome limitations-such as the lack of identification of robust targets and poor clinical efficacy-that hamper cancer drug development. Here we performed genome-scale CRISPR-Cas9 screens in 324 human cancer cell lines from 30 cancer types and developed a data-driven framework to prioritize candidates for cancer therapeutics. We integrated cell ... More
ZFP30 promotes adipogenesis through the KAP1-mediated activation of a retrotransposon-derived Pparg2 enhancer.
Authors:Chen W, Schwalie PC, Pankevich EV, Gubelmann C, Raghav SK, Dainese R, Cassano M, Imbeault M, Jang SM, Russeil J, Delessa T, Duc J, Trono D, Wolfrum C, Deplancke B
Journal:Nat Commun
PubMed ID:31000713
'Krüppel-associated box zinc finger proteins (KZFPs) constitute the largest family of mammalian transcription factors, but most remain completely uncharacterized. While initially proposed to primarily repress transposable elements, recent reports have revealed that KFZPs contribute to a wide variety of other biological processes. Using murine and human in vitro and in ... More
Genome-wide mapping reveals that deoxyuridine is enriched in the human centromeric DNA.
Authors:Shu X, Liu M, Lu Z, Zhu C, Meng H, Huang S, Zhang X, Yi C
Journal:Nat Chem Biol
PubMed ID:29785056
'Uracil in DNA can be generated by cytosine deamination or dUMP misincorporation; however, its distribution in the human genome is poorly understood. Here we present a selective labeling and pull-down technology for genome-wide uracil profiling and identify thousands of uracil peaks in three different human cell lines. Surprisingly, uracil is ... More
CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome.
Authors:Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy TE, Gersbach CA
Journal:Nat Biotechnol
PubMed ID:28369033
Large genome-mapping consortia and thousands of genome-wide association studies have identified non-protein-coding elements in the genome as having a central role in various biological processes. However, decoding the functions of the millions of putative regulatory elements discovered in these studies remains challenging. CRISPR-Cas9-based epigenome editing technologies have enabled precise perturbation ... More
Optogenetic control of kinetochore function.
Authors:Zhang H, Aonbangkhen C, Tarasovetc EV, Ballister ER, Chenoweth DM, Lampson MA
Journal:Nat Chem Biol
PubMed ID:28805800
Kinetochores act as hubs for multiple activities during cell division, including microtubule interactions and spindle checkpoint signaling. Each kinetochore can act autonomously, and activities change rapidly as proteins are recruited to, or removed from, kinetochores. Understanding this dynamic system requires tools that can manipulate kinetochores on biologically relevant temporal and ... More
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