Collagen I, Coated Plate, 24 well

Enzymes that can be used include: Gibco TrypLE Express Enzyme, Gibco TrypLE Select Enzyme, or Gibco StemPro Accutase Cell Dissociation Reagent.

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The following cell types are commonly cultured in collagen I:

- Endothelial cells (including HUVEC)
- Fibroblasts
- Hepatocytes
- Chondrocytes
- Osteoclasts
- Osteoblasts
- Keratinocytes
- Corneal epithelium

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The key applications include the following:

- Collagen I induces microvascular endothelial cells to adopt a spindle-shaped morphology, in vitro, and to align into solid cord-like assemblies. Vascular endothelial cells can also form vessel-like, tubular structures when cultured on collagen I. Collagen I can be used for in vitro angiogenesis assays.
- Breast cancer stem cells can undergo differentiation when cultured on collagen I.
- Collagen I has been used for the culture of primary colon carcinoma cell lines; mouse liver progenitor cells have been cultured in 3D collagen I and rat pancreatic islets.
- Collagen I can act as a barrier in cell invasion assays, and has been used to study cell adhesion.

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Gibco Geltrex Matrix and collagen (rat tail and bovine) can be used as either a coating solution or a 3D gel matrix. CELLstart Substrate was developed to be used as a xeno-free coating matrix for only ESC applications (as a substitute for Gibco Geltrex Matrix or Matrigel Matrix, which are of animal origin). AlgiMatrix Matrix is a 3D scaffold-type matrix that does not support cell attachment but does provide a good environment for growing spheroids that can be easily harvested for downstream applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.