Hibernate™-E Medium
Hibernate™-E Medium
인용 및 참조 문헌 (1)
Gibco™

Hibernate™-E Medium

Hibernate Medium is a CO2-independent nutrient medium for the maintenance of neural cells, tissue, and tissue slices. Hibernate-E Medium is자세히 알아보기
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카탈로그 번호수량
A1247601500 mL
카탈로그 번호 A1247601
제품 가격(KRW)
208,000
Online offer
Ends: 31-Mar-2026
231,000
할인액 23,000 (10%)
Each
카트에 추가하기
수량:
500 mL
제품 가격(KRW)
208,000
Online offer
Ends: 31-Mar-2026
231,000
할인액 23,000 (10%)
Each
카트에 추가하기
Hibernate Medium is a CO2-independent nutrient medium for the maintenance of neural cells, tissue, and tissue slices. Hibernate-E Medium is optimized for embryonic cells or tissue in ambient carbon dioxide levels.

Hibernate Medium enables neurons to be maintained in ambient CO2 levels for up to 48 hours. This provides scientists with greater convenience and control while running bench-top live cell experiments, such as microscopy, flow cytometry, and other physiology studies. Hibernate Medium also allows for the preservation of viable brain tissue before processing for up to a month with refrigeration and maintenance of tissue slices.

Hibernate Medium may be combined with B-27 Supplements to yield a complete medium.

For other applications, we provide alternative formulations of Hibernate Medium and B-27 Supplements.

For established protocols using Hibernate Medium, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Neural Stem Cells
제품라인Hibernate
제품 유형Stem Cell Media
수량500 mL
유통 기한24 Months
배송 조건Ambient
테스트됨Cytotoxicity, Endotoxin, Sterility, pH, Osmolality
분류Serum-free
Culture TypeMammalian Cell Culture
형태Liquid
Serum LevelSerum-free
멸균Sterile
첨가제 포함Phenol Red
Unit SizeEach
구성 및 보관
Store in refrigerator (2–8°C) and protect from light.

자주 묻는 질문(FAQ)

Can the Hibernate-E Medium (Cat. No. A1247601) be used for plating and growing mouse primary hippocampal neurons?

Hibernate-E medium (Cat. No. A1247601) is designed for the maintenance of embryonic neural tissue and is suitable for short-term maintenance of cultured rat neurons at 37°C in ambient CO2 (up to 2 days) and long-term storage of viable brain tissue at 2 to 8°C (up to a month). However, it is not recommended for the initial plating and growing of neurons.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What medium can be used to maintain/manipulate neurons at ambient CO2?

We offer Gibco Hibernate Medium for this purpose. When supplemented with Gibco B-27 Supplement and Gibco GlutaMAX-1 Supplement, this medium can allow for the manipulation of neurons at ambient CO2 for at least 48 hours while retaining their viability, and preserve viable brain tissue for up to a month when stored at 4 degrees C. Two Hibernate media are offered:

- Gibco Hibernate-A Medium (Cat. No. A1247501) is formulated for postnatal neurons.
- Gibco Hibernate-E Medium (Cat. No. A1247601) is formulated for embryonic neurons.

These two media have the same formulation and differ only in osmolality.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Long-term hibernation of human fetal striatal tissue does not adversely affect its differentiation in vitro or graft survival: implications for clinical trials in Huntington's disease.
Authors:Hurelbrink CB, Tyers P, Armstrong RJ, Dunnett SB, Barker RA, Rosser AE
Journal:Cell Transplant
PubMed ID:14653616
Transplantation of human fetal CNS tissue is a promising therapy for neurodegenerative conditions such as Huntington's disease (HD), but its widespread adoption is limited by restricted tissue availability. One method of overcoming this problem would be to store the tissue in hibernation medium, an approach that we reported previously for ... More