Lipofectamine™ LTX Reagent with PLUS™ Reagent
Lipofectamine™ LTX Reagent with PLUS™ Reagent
Invitrogen™

Lipofectamine™ LTX Reagent with PLUS™ Reagent

Lipofectamine™ LTX Reagent은 transfection이 어려운 세포의 유전자 발현 연구에 새로운 첨단 해결책을 제공합니다. 어렵고 민감한 일차 세포주의 transfection에는 Lipofectamine™ LTX자세히 알아보기
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카탈로그 번호수량
A126210.1 mL
153380300.3 mL
153381001 mL
카탈로그 번호 A12621
제품 가격(KRW)
121,000
Precio exclusivo en nuestra web
Ends: 31-Dec-2025
134,000
할인액 13,000 (10%)
Each
카트에 추가하기
수량:
0.1 mL
제품 가격(KRW)
121,000
Precio exclusivo en nuestra web
Ends: 31-Dec-2025
134,000
할인액 13,000 (10%)
Each
카트에 추가하기
Lipofectamine™ LTX Reagent은 transfection이 어려운 세포의 유전자 발현 연구에 새로운 첨단 해결책을 제공합니다. 어렵고 민감한 일차 세포주의 transfection에는 Lipofectamine™ LTX Reagent의 효율성(efficiency)과 편이성(convenience), 관용성(gentleness)을 따라올 만한 플라스미드 DNA 특이 transfection reagent가 없습니다. Lipofectamine™ LTX Reagent는 100% 동물유래성분이 없는 성분들로부터 합성된 제품으로 연구실험이나 세포주에서 BSE나 바이러스같은 동물원성 질병의 유무를 쉽게 검사할 수 있습니다. 원산지 증명서(C/O)는 기술 지원부에 연락해 받을 수 있습니다. Lipofectamine™ LTX Reagent의 특징:
• 우수한 전달력 - 어려운 일차 세포를 포함해 다양한 세포 유형의 transfection에 높은 효율성 보장
• 우수한 성능 - 높은 transfection 효율과 최대(>90%) 세포 생활성 제공
• 최적화 필요성 절감 - 대부분의 세포주에 신속하고 단순한 protocol 제공

일차 세포 transfection 효율 향상

일차 세포는 transfection이 어려운 경우가 많았습니다. Lipofectamine™ LTX Reagent과 PLUS™ Reagent을 함께 사용해 일차 신경전구세포 등의 일차 세포 transfection 문제를 해결합니다(그림 1).

transfection이 어려운 세포에 우수한 전달

RAW267.3 포식세포에서 높은 수준의 단백질 발현을 달성해야 하는 경우 Lipofectamine™ LTX Reagent이 경쟁사 transfection 시약보다 우수한 성능을 보입니다(그림 2). 이전에 transfection이 어려웠던 세포에서 high-level transgene expression을 달성하여 이전에 구하지 못했던 결과를 얻을 수 있습니다.

폭넓은 세포주 호환성

어려운 일차 세포 transfection 성능 외에도 Lipofectamine™ LTX Reagent는 다른 transfection 시약에 민감한 세포주를 포함해 확립된 세포주 transfection에 다용도로 사용할 수 있습니다. 이런 세포 유형에 권장되는 transfection 조건은 www.invitrogen.com/transfection에서 찾아볼 수 있습니다.

간편하고 신속한 혈청 호환 protocol

Lipofectamine™ LTX Reagent은 transfection 화학물 제거나 transfection 후 배지 변경/추가가 필요하지 않은 간단한 protocol을 제공합니다. 직접 작업하는 시간을 줄이고 빨리 결과를 얻으세요.

이 제품은 진단용으로만 사용가능합니다. 동물 또는 사람의 치료 또는 진단용으로 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
분류Animal Origin-Free
용도(애플리케이션)Transfection
고처리량 호환성High-throughput Compatible
제품라인Lipofectamine
제품 유형LTX Reagent
수량0.1 mL
RNAi 유형ShRNA, miR
혈청 호환 가능Yes
배송 조건Wet Ice
셀 유형Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
형식6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
샘플 종류Plasmid DNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
Unit SizeEach
구성 및 보관
Each unit contains one vial of Lipofectamine™ LTX Reagent (0.1 mL) and one vial of Plus Reagent (40 μl). Store at 2–8°C.

자주 묻는 질문(FAQ)

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

Is it necessary to use serum-free medium during lipid transfection?

It is not necessary to use serum-free medium during lipid transfection. However, it is critical to form the lipid:nucleic acid complex in the absence of serum, because proteins can interfere with complex formation. Once the complexes are formed, they can be added to cells in serum-containing medium. For optimal results with Lipofectin Transfection Reagent, we recommend performing transfection in medium without serum.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.