The GeneArt™ Linear pUC19L Vector is a ready-to-use, linear version of the popular cloning vector pUC19. The pUC19L plasmid (2.7 Kbp), is specifically designed as a cloning and DNA assembly vector for the GeneArt™ Seamless Cloning and Assembly Kit (cat # A13288). The vector is manufactured by digesting supercoiled pUC19 plasmid with the restriction enzymes PstI and KpnI, and tested for cloning of up to 4 DNA fragments.
Some of the key features and elements of the pUC19L vector include: • lacZα gene allows easy blue⁄white colony screening for selection of recombinants • pUC origin of replication (high copy vector) • Ampicillin resistance for selection in E. coli • 6 convenient and validated restriction sites flanking the cloning region • Tested for functionality by assembly with 4 DNA fragments using the GeneArt™ Seamless Cloning and Assembly Kit
For Research Use Only. Not for use in diagnostic procedures.
사양
제품 유형
Cloning Vector
리포터 유전자
Beta-Gal (lacZ)
항생제 내성 박테리아
Ampicillin (AmpR)
복제 개시점
pUC Origin
배송 조건
Dry Ice
크기 범위
13 kb total (vector plus all inserts)
클로닝 방법
Seamless Cloning
농도
50 ng/mL
수량
Up to 4 Fragments
벡터
Seamless Cloning Vectors
제품라인
GeneArt™
구성 및 보관
The product contains 5 vials of linear pUC19L vector at a concentration of 50ng⁄ml. Each vial contains enough plasmid for 4 cloning reactions using the GeneArt™ Seamless Cloning and Assembly Kit (A13288 - must be purchased separately).
Store the product at -80°C.
The vector is guaranteed stable for 6 months when properly stored.