CTS™ Synth-a-Freeze™ Medium
CTS™ Synth-a-Freeze™ Medium
인용 및 참조 문헌 (6)
Gibco™

CTS™ Synth-a-Freeze™ Medium

Cell Therapy Systems Synth-a-Freeze™ CTS™ media는 화학적으로 규정된 protein-free 액체 동결보존 배지로 줄기세포를 포함한 다양한 유형의 포유류 세포를 동결 및자세히 알아보기
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카탈로그 번호수량
A137130150 mL
카탈로그 번호 A1371301
제품 가격(KRW)
181,000
Online offer
Ends: 31-Dec-2025
201,000
할인액 20,000 (10%)
Each
카트에 추가하기
수량:
50 mL
제품 가격(KRW)
181,000
Online offer
Ends: 31-Dec-2025
201,000
할인액 20,000 (10%)
Each
카트에 추가하기

Cell Therapy Systems Synth-a-Freeze™ CTS™ media는 화학적으로 규정된 protein-free 액체 동결보존 배지로 줄기세포를 포함한 다양한 유형의 포유류 세포를 동결 및 보관할 수 있도록 만들어졌습니다. Synth-a-Freeze™ CTS™ media는 항생제, 항진균제, 호르몬, 성장인자, 혈청, 단백질이 포함되지 않은 최적의 formulation으로 HEPES 및 sodium bicarbonate가 buffer로 이용됩니다.

  • 다양한 초대 배양 세포 및 줄기 세포의 동결보존에 이용
  • 해동된 배지의 영상 4도에서도 안정적 보관 및 다양한 해동 protocol과의 호환성으로 시간과 노력 절약
  • cGMP에 부합하는 화학적으로 규정된 무단백 조성으로 임상 연구에 바로 사용 가능

다양한 세포 유형의 동결보존

Cell Therapy Systems Synth-a-Freeze™ CTS™ media는 인간 배아줄기세포, 신경줄기세포(Ref 1), 간엽성 줄기세포, 각질형성세포(Ref 2)등의 다양한 세포 유형의 동결보전을 위한 자사 표준, 혈청 함유 배지와 유사하게 작용합니다. 인간 상피 멜라닌 세포의 동결건조에는 Synth-a-Freeze™ media 사용이 권장되지 않습니다.

현행 동결 protocol에의 통합이 용이

Synth-a-Freeze™ CTS™ media는 모든 표준 동결 protocol에 사용할 수 있습니다. 해동한 Cell Therapy Systems Synth-a-Freeze™ CTS™ media를 분리된 펠렛 세포(pelleted)에 넣고 분주하여 동결시킵니다. 최적의 결과를 위해 속도 제어 냉동기의 사용이 권장됩니다.

임상 연구로의 손쉬운 전환

Gibco™ Cell Therapy Systems (CTS™) 브랜드는 배지, 시약, 성장인자, 효소, 세포 선택/확장 시약, 장치 등 세포 치료 어플리케이션에 필요한 다양한 제품을 제공합니다. Gibco™ Cell Therapy Systems (CTS™) 제품의 특징:

  • 시약 적격성 검사 부담 감소
  • 해당 성적서 및 원산지 증명서 포함
  • 편리하게 본사 약물 마스터 파일(DMF) 열람 가능

연구용 또는 세포, 유전자, 조직 기반 제품의 제조에 사용가능합니다.

주의: 사람이나 동물에게 직접 투여할 수 없습니다.

참고문헌:

  1. S. Shin and M. Vemuri. Culture and Differentiation of Human Neural Stem Cells. L.C. Doering (ed). Protocols for Neural Cell Culture. Springer Protocol Handbooks.
  2. S. Lemaire, K. Kosowska-Shick, P. Appelbaum, G. Verween, P. Tulkens, and F. Van Bambeke. Cellular Pharmacodynamics of the Novel Biaryloxazolidinone Radezolid: Studies with Infected Phagocytic and Nonphagocytic cells, Using Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, and Legionella pneumophila. Antimicrob Agents Chemother 54(6) 2010: 2549-2559.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Various (including types of human Embryonic Stem Cells, Neural Stem Cells, Mesenchymal Stem Cells, and Keratinocytes)
용도(애플리케이션)Cell and Gene Therapy Research, Development, and Manufacturing
형식Bottle
제조 품질ISO 13485, MDSAP, FDA-registered, 21 CFR 820
제품 유형Cryopreservation Medium
수량50 mL
유통 기한18 months from date of manufacture
배송 조건Dry Ice
분류Animal Origin-free
형태Liquid
멸균Sterile-filtered
첨가제 포함DMSO (10%)
Unit SizeEach
구성 및 보관
Store at –20°C.

자주 묻는 질문(FAQ)

What is CTS?

The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

If the Synth-a-Freeze Cryopreservation Medium is not suitable for melanocytes, how should I freeze melanocytes?

We recommend using DMEM plus 10% FBS and 10% DMSO, or the Recovery Cell Culture Freezing Medium. You can try the same general recommendations for freezing cells, just changing the freezing solution.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is there a Drug Master File for the Synth-a-Freeze Cryopreservation Medium?

There isn't a Drug Master File, but there is a Device Master File on file with the FDA. Please contact our Licensing team at outlicensing@thermofisher.com in order to obtain instructions for referencing this Device Master File.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What can I use Synth-a-Freeze Cryopreservation Medium for?

Synth-a-Freeze Cryopreservation Medium can be used with any standard freezing protocol. It offers performance comparable to that of our standard, serum-containing cryopreservation medium for cyropreserving a variety of cell types including human keratinocytes, embryonic stem cells, neural stem cells, and mesenchymal stem cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Was the tissue cryopreserved prior to isolation of primary cells?

No, the tissue was not cryopreserved.

View all CTS™ Synth-a-Freeze™ Medium FAQs

인용 및 참조 문헌 (6)

인용 및 참조 문헌
Abstract
Serum-free suspension culturing of human cells: adaptation, growth, and cryopreservation.
Authors:Biaggio RT, Abreu-Neto MS, Covas DT, Swiech K
Journal:Bioprocess Biosyst Eng
PubMed ID:25822314
Human cell lines have attracted great interest because they are capable of producing glycosylated proteins that are more similar to native human proteins, thereby reducing the potential for immune responses. However, these cells have not been extensively characterized and cultured under serum-free suspension conditions. In this work, we describe the ... More
Amnion epithelial cell isolation and characterization for clinical use.
Authors:Murphy S, Rosli S, Acharya R, Mathias L, Lim R, Wallace E, Jenkin G
Journal:Curr Protoc Stem Cell Biol
PubMed ID:20373516
Human amnion epithelial cells (hAECs) are a heterologous population positive for stem cell markers; they display multilineage differentiation potential, differentiating into cells of the endoderm (liver, lung epithelium), mesoderm (bone, fat), and ectoderm (neural cells). They have a low immunogenic profile and possess potent immunosuppressive properties. Hence, hAECs may be ... More
Cellular pharmacodynamics of the novel biaryloxazolidinone radezolid: studies with infected phagocytic and nonphagocytic cells, using Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, and Legionella pneumophila.
Authors:Lemaire S, Kosowska-Shick K, Appelbaum PC, Verween G, Tulkens PM, Van Bambeke F
Journal:Antimicrob Agents Chemother
PubMed ID:20385852
Radezolid is a novel biaryloxazolidinone in clinical development which shows improved activity, including against linezolid-resistant strains. In a companion paper (29), we showed that radezolid accumulates about 11-fold in phagocytic cells, with approximately 60% of the drug localized in the cytosol and approximately 40% in the lysosomes of the cells. ... More
Rapid and Efficient Generation of Transgene-Free iPSC from a Small Volume of Cryopreserved Blood.
Authors:Zhou H, Martinez H, Sun B, Li A, Zimmer M, Katsanis N, Davis EE, Kurtzberg J, Lipnick S, Noggle S, Rao M, Chang S
Journal:
PubMed ID:25951995
Human peripheral blood and umbilical cord blood represent attractive sources of cells for reprogramming to induced pluripotent stem cells (iPSCs). However, to date, most of the blood-derived iPSCs were generated using either integrating methods or starting from T-lymphocytes that have genomic rearrangements thus bearing uncertain consequences when using iPSC-derived lineages ... More
Biological impact of xeno-free chemically defined cryopreservation medium on amniotic epithelial cells.
Authors:Miki T, Wong W, Zhou E, Gonzalez A, Garcia I, Grubbs BH
Journal:Stem Cell Res Ther
PubMed ID:26758986
'Amnion-derived stem cells have been proposed for cell replacement therapy and tissue regeneration. An easily accessible cell source, the placenta, allows us to potentially establish a bio-bank of cells for immunotype matched clinical applications. Several xeno-free (XF) cryopreservation media are currently available for pluripotent stem cells, however, these media have ... More
View all CTS™ Synth-a-Freeze™ Medium citations