Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix
DISCONTINUATION NOTICE: Geltrex™ Reduced Growth Factor Matrices won't be restocked — switch to NEW Geltrex™ Flex Reduced Growth Factor Matrices with equivalent performance, expanded vial sizes, and an added organoid-qualified option.
Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix
인용 및 참조 문헌 (14)
Gibco™

Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix

Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS)자세히 알아보기
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카탈로그 번호수량
A14132025 mL
A14132011 mL
NOTICE: This product will no longer be restocked — switch now to the replacement product Geltrex™ Flex Reduced Growth Factor matrix, delivering the same trusted performance in expanded vial sizes, along with a new organoid qualified matrix choice.
카탈로그 번호 A1413202
현재 제품 선택
제품 가격(KRW)
379,000
Online offer
Ends: 31-Dec-2025
421,000
할인액 42,000 (10%)
Each
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Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation. The major components of Geltrex™ matrix include laminin, collagen IV, entactin, and heparin sulfate proteoglycans. It is ideal for the promotion and maintenance of differentiated phenotypes in a variety of cell cultures including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC). Geltrex™ matrix offers, on average, a 2-fold reduction in several growth factors. As a result, analysis of specific growth factor effects in applications requiring basement membrane extract can be carried out in a controlled and reproducible format.

Key Features of Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix:

• Free of lactose dehydrogenase elevating virus (LDEV) making it ideal for all types of cell culture and mouse in vivo applications
• Consistent protein concentration lot-to-lot helps reduce the need to screen lots
• Protocols for various applications, including tube formation assays, are available

Gain Reliability and Consistency in Your hESC Studies
Recent advances in processing EHS tumor cells have resulted in virus free basement membrane extracts. Every lot is tested by RT-PCR methods so you can now have confidence that every lot of Geltrex™ is virus free. Additionally, our consistent manufacturing process helps ensure a protein concentration with very little variation from lot-to-lot, averaging 15 mg/ml so you can have confidence in every purchase.

Investigate Angiogenesis with Validated Gibco™ Protocols
There are many applications for Geltrex™ LDEV-Free reduced growth factor basement membrane matrix, each requiring different thicknesses and concentrations. In general, a protein concentration of >5 mg/ml is used for differentiation studies of primary cells. Geltrex™ LDEV-Free reduced growth factor basement membrane matrix has been used to develop tube formation assay protocols using Gibco™ primary cells and specialty media. See this and other angiogenesis assay protocols
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Variety of cultured cells, including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC)
농도12 to 18 mg/mL
엔도톡신 수준Low
형식Tube(s)
제품라인Gibco
제품 유형Basement Membrane Matrix
순도 또는 품질 등급Cell Culture Grade
수량5 mL
유통 기한36 months
배송 조건Dry Ice
종Mouse
분류Reduced Growth Factor
Culture TypeAdherent Cell Culture
형태Frozen
Serum LevelSerum-free
멸균Sterile
첨가제 없음No Phenol Red
Unit SizeEach
구성 및 보관
Store at -20°C.

자주 묻는 질문(FAQ)

What growth factors are reduced in the Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413202)?

Although the standard basement membrane extract (BME) contains growth factors such as EGF, IGF‐1, PDGF and TGF‐beta, the reduced growth factor basement membrane extract product (Geltrex matrix) contains below detectable levels of EGF, PDGF and NGF. It still has some TGF‐beta (about 2 ng/mL) and 3 times less IGF‐1 on average than regular BME, hence the designation "reduced". Neither standard nor reduced BME contain NGF or FGF‐2.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center

What volume do you recommend for thin coating 35 mm dishes with Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413201, A1413202) or Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301, A1413302)?

We recommend thin (non-gelling) coating 35 mm dishes with 1.5 mL of Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413201, A1413202) or Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301, A1413302) prediluted 1/100 in DMEM/F-12 medium (1% final concentration).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I refreeze and reuse Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix after having used it in an experimental setup?

Yes, you can refreeze and reuse Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix. However, you should avoid multiple freeze/thaw cycles. hence, we would recommend the following:

  1. Thaw Geltrex Matrix in a refrigerator overnight at 2-8 degrees C.
  2. Mix Geltrex Matrix solution by slowly pipetting up and down. Be careful not to introduce air bubbles.
  3. If not using up the complete solution, prepare multiple aliquots, either of the original solution or the diluted one, and freeze.


Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I characterize human embryonic stem ( ES) cells?

Human ES cells are generally characterized by their typical morphology (they grow as tightly packed clusters of small cells with high ratio of nucleus to cytoplasm); surface marker expression; RT-PCR detection of stem cell-specific gene expression (such as Oct3/4, Sox2, and Nanog); alkaline phosphatase staining, and telomerase activity assay. The most commonly used ES specific surface markers include stage-specific embryonic antigens SSEA-3 and SSEA-4 for human ES cells. Other ES-specific surface antigens also include TRA-1-60 and TRA-1-81. (Science 282:1145 (1998).

How are human embryonic stem (ES) cells derived?

Human ES cells are derived from human blastocyst inner cell masses, isolated by immunosurgery with rabbit antiserum to BeWO cells (a human trophoblast cell line) (Science 282:1145 (1998)).

View all Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix, 5 mL FAQs

인용 및 참조 문헌 (14)

인용 및 참조 문헌
Abstract
Microgels on-demand.
Authors:Eydelnant IA, Betty Li B, Wheeler AR
Journal:
PubMed ID:24566526
'Three-dimensional (3D) hydrogel structures are finding use in fundamental studies of self-assembly, rheology, and 3D cell culture. Most techniques for 3D hydrogel formation are ''single pot'', in which gels are not addressable after formation. For many applications, it would be useful to be able to form arrays of gels bearing ... More
Lumen Formation Is an Intrinsic Property of Isolated Human Pluripotent Stem Cells.
Authors:Taniguchi K, Shao Y, Townshend RF, Tsai YH, DeLong CJ, Lopez SA, Gayen S, Freddo AM, Chue DJ, Thomas DJ, Spence JR, Margolis B, Kalantry S, Fu J, O'Shea KS, Gumucio DL
Journal:
PubMed ID:26626176
'We demonstrate that dissociated human pluripotent stem cells (PSCs) are intrinsically programmed to form lumens. PSCs form two-cell cysts with a shared apical domain within 20 hr of plating; these cysts collapse to form monolayers after 5 days. Expression of pluripotency markers is maintained throughout this time. In two-cell cysts, ... More
Epithelial-mesenchymal transitioned circulating tumor cells capture for detecting tumor progression.
Authors:Satelli A, Mitra A, Brownlee Z, Xia X, Bellister S, Overman MJ, Kopetz S, Ellis LM, Meng QH, Li S
Journal:
PubMed ID:25516888
This study aimed to detect cell-surface vimentin (CSV) on the surface of epithelial-mesenchymal transitioned (EMT) circulating tumor cells (CTC) from blood of patients with epithelial cancers. ... More
Induced pluripotent stem cell-derived mesenchymal stem cell seeding on biofunctionalized calcium phosphate cements.
Authors:TheinHan W, Liu J, Tang M, Chen W, Cheng L, Xu HH
Journal:
PubMed ID:24839581
Induced pluripotent stem cells (iPSCs) have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells (iPSC-MSCs), and investigate iPSC-MSC proliferati on and osteogenic differentiation on calcium phosphate cement (CPC) containing biofunctional agents for the first time. Human iPSCs ... More
Neural Differentiation of Spheroids Derived from Human Induced Pluripotent Stem Cells-Mesenchymal Stem Cells Coculture.
Authors:Song L, Tsai AC, Yuan X, Bejoy J, Sart S, Ma T, Li Y
Journal:Tissue Eng Part A
PubMed ID:29160172
'Organoids, the condensed three-dimensional (3D) tissues emerged at the early stage of organogenesis, are a promising approach to regenerate functional and vascularized organ mimics. While incorporation of heterotypic cell types, such as human mesenchymal stem cells (hMSCs) and human induced pluripotent stem cells (hiPSCs)-derived neural progenitors aid neural organ development, ... More
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