Alkaline Phosphatase Live Stain
Alkaline Phosphatase Live Stain
인용 및 참조 문헌 (3)
Invitrogen™

Alkaline Phosphatase Live Stain

Alkaline Phosphatase Live Stain은 사용자가 만능줄기세포 (pluripotent stem cell, PSC)를 구분하여 염색할 수 있는 줄기세포 영상검사 제품입니다. 이 dye는 세포자세히 알아보기
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카탈로그 번호수량
A1435350 μl
카탈로그 번호 A14353
제품 가격(KRW)
471,000
キャンペーン価格
Ends: 31-Mar-2026
523,000
할인액 52,000 (10%)
Each
카트에 추가하기
수량:
50 μl
제품 가격(KRW)
471,000
キャンペーン価格
Ends: 31-Mar-2026
523,000
할인액 52,000 (10%)
Each
카트에 추가하기
Alkaline Phosphatase Live Stain은 사용자가 만능줄기세포 (pluripotent stem cell, PSC)를 구분하여 염색할 수 있는 줄기세포 영상검사 제품입니다. 이 dye는 세포 투과성 alkaline phosphatase (AP) 형광 기질로 세포에 대한 독성이 없으며, 두 시간에 걸쳐 확산됩니다. Alkaline Phosphatase Live Stain은 DMSO에 들어있는 500X fluorescein 기반 dye 50 μL로 이루어져 24-well 플레이트 2개, 6 cm 배양용기 4개, 또는 10 cm 배양용기를 염색하는데 충분합니다.

Alkaline Phosphatase Live Stain의 장점:

• background와 확실히 구분되는 강력한 신호로 만능줄기세포 확인
• 대개 20분 이내에 PSC 염색
• 세포 생활성을 유지시키는 첫 시판 alkaline phosphatase stain 사용

Alkaline Phosphatase 만능줄기세포 검출 방법
AP는 미분화 배아줄기세포(ESC), 유도 만능줄기세포(iPSC), 배아 생식세포(EGC) 등을 포함한 PSC의 표현형 표지자입니다. AP는 대부분의 세포 유형에서 발현되지만, PSC에서는 그 발현이 크게 상승됩니다. 따라서 AP 염색을 이용하여 PSC를 다르게 염색하여 feeder로 사용하는 마우스 배아섬유모세포(mouse embryonic fibroblast, MEF)와 reprogramming 실험에 흔히 사용되는 모체 섬유모세포를 쉽게 구분합니다.

소중한 집락 손실이 없는 다목적 스크리닝
Alkaline Phosphatase Live Stain은 염색에도 줄기세포의 생활성이 유지되기 때문에 재프로그래밍 workflow의 초기 단계에서 집락을 선별하는데 적합합니다. 이는 또한 미분화 세포 확인을 위한 음성 선택 도구로 이후 단계에서도 사용될 수 있습니다.

신속한 세포 염색
Alkaline Phosphatase Live Stain은 쉽고 비영구적이며, 세포가 살아있는 protocol을 사용하여 실험에서 PSC를 확인합니다. 배지에 dye를 희석하여 세포에 적용한 후 부드럽게 세척하기만 하면 세포의 형광 영상검사에 대한 준비가 끝납니다.

이 제품은 연구용으로만 사용가능합니다. 진단 절차에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Stem Cells (iPS - Induced Pluripotent Stem), Stem Cells (Embryonic)
검출 방법Fluorescence
염료 유형Classic Dyes
형태Liquid
수량50 μl
연구 카테고리Differentiation
배송 조건Dry Ice
세포하위구조 국지화Cell Membranes & Lipids
기질 유형AP (Alkaline Phosphatase) Substrate
색상Green
용도(애플리케이션)Viability Assay
용도 (장비)Floid Cell Imaging System, Fluorescence Microscope
제품 유형Alkaline Phosphatase Live Stain
Unit SizeEach
구성 및 보관
Contains 1 amber vial. Store at -20°C.

자주 묻는 질문(FAQ)

How can I prevent poor or dim staining when using Alkaline Phosphatase Live Stain?

Serum or serum replacement components in the growth medium may cause background and this can result in poor or dim staining. After the removal of the growth medium, gently wash the culture with pre-warmed DMEM/F-12 (Cat. No. 10565-018) for 5 minutes. Aspirate and repeat once before adding the AP Live Stain. To further decrease background staining, perform 3 separate washes of 5 minutes each with DMEM/F-12 for a total of 15 minutes following the staining, and visualize immediately.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should Alkaline Phosphatase Live Stain be stored?

Store AP Live Stain at -20 degrees C in the freezer and thaw at room temperature. Avoid repeated freeze/thaw cycles and aliquot the AP Live Stain into smaller volumes if necessary. Always protect from light and avoid extended exposure at room temperature and atmospheric conditions.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use Alkaline Phosphatase Live Stain in my reprogramming experiments?

AP Live Stain is ideal for screening colonies during early stages of reprogramming since it selectively stains PSCs while maintaining cell viability. It can be used in later stages of reprogramming to identify undifferentiated cells for the selection of iPSCs for further cultivation. AP Live Stain was developed specifically for use on live cultures for cell maintenance and is qualified to be free of mycoplasma and bioburden, and exhibits extremely low endotoxin levels.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long should I wait before using Alkaline Phosphatase Live stain on my cells again?

Colonies may be restained as early as 24 hours after the initial staining. It is recommended that cells are allowed to recover properly to obtain consistent results.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How many times can the same cells be stained with Alkaline Phosphatase Live Stain?

AP Live Stain can be used daily throughout the life of a culture without any adverse effects.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Alkaline Phosphatase Live Stain FAQs

인용 및 참조 문헌 (3)

인용 및 참조 문헌
Abstract
Novel Live Alkaline Phosphatase Substrate for Identification of Pluripotent Stem Cells.
Authors:Singh U, Quintanilla RH, Grecian S, Gee KR, Rao MS, Lakshmipathy U,
Journal:Stem Cell Rev
PubMed ID:22426885
Characterization of pluripotent stem cells.
Authors:Martí M, Mulero L, Pardo C, Morera C, Carrió M, Laricchia-Robbio L, Esteban CR, Izpisua Belmonte JC,
Journal:Nat Protoc
PubMed ID:23306458
Characterization of pluripotent stem cells is required for the registration of stem cell lines and allows for an impartial and objective comparison of the results obtained when generating multiple lines. It is therefore crucial to establish specific, fast and reliable protocols to detect the hallmarks of pluripotency. Such protocols should ... More
Immune Defense Protein Expression in Highly Purified Mouse Lung Epithelial Cells.
Authors:Sinha M, Lowell CA
Journal:Am J Respir Cell Mol Biol
PubMed ID:26574781
Lung epithelial cells play critical roles in initiating and modulating immune responses during pulmonary infection or injury. To better understand the spectrum of immune response-related proteins present in lung epithelial cells, we developed an improved method of isolating highly pure primary murine alveolar type (AT) II cells and murine tracheal ... More