Expi293F™ Cells
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Expi293F™ Cells
Gibco™

Expi293F™ Cells

Expi293F human cells are derived from the 293 cell line and are a core component of the Expi293 Expression System.
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카탈로그 번호수량
A145286 x 1 mL
A145271 mL
카탈로그 번호 A14528
제품 가격(KRW)
3,128,000
온라인 행사
Ends: 31-Dec-2025
3,475,000
할인액 347,000 (10%)
Each
카트에 추가하기
수량:
6 x 1 mL
제품 가격(KRW)
3,128,000
온라인 행사
Ends: 31-Dec-2025
3,475,000
할인액 347,000 (10%)
Each
카트에 추가하기

Expi293F human cells are derived from the 293 cell line and are a core component of the Expi293 Expression System. They are maintained in suspension culture and will grow to high density in Expi293 Expression Medium. Expi293F cells are highly transfectable and generate superior protein yields compared to standard 293 cell lines in transient protein expression.

Expi293F Cells attributes include:

  • Suspension culture cells pre-adapted to Expi293 Expression Medium
  • Demonstrated higher protein expression levels than other 293 cells adapted to Expi293 Expression Medium
  • Exhibit more rapid cell growth and higher culture viabilities than standard suspension-adapted 293 cells
  • Consistent transient expression performance of Expi293F cells for over 30 passages beyond thaw
  • Also available from a cGMP bank (Cat. No. 100044202)

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Expi293 Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 Expression Medium. The six-vial multipack eliminates the need to expand and rebank the cells in the lab.

Cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 mL in multiwell plates to >10 L in bioreactors. Protein harvest can be performed at 2–7 days post-transfection, depending on the protein expressed. For expression of recombinant antibodies, harvest is typically at 5–7 days post-transfection. There is no need for culture medium supplementation or batch feeding during expression.

For Research Use Only. Not for use in diagnostic procedures.
사양
미디어 권장 사항Expi293™ Expression Medium
셀 수1 x 107
제품라인Expi293
제품 유형293-F Cells
수량6 x 1 mL
세포주Expi293F
셀 유형Kidney (Embryonic)
Culture TypeSuspension Cell Culture
형태Cryopreserved
종Human
Unit SizeEach
구성 및 보관
1 x 107 cells/mL, store in liquid nitrogen

자주 묻는 질문(FAQ)

Can I leave my Expi293F culture expressing for more than 7 days?

The growth and expression characteristics of Expi293F cells are such that, by 7 days post-transfection, the culture medium should be close to being spent and maximal protein expression should have already been achieved. Continued incubation will result in a large decrease in cell culture viability.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How many days should I wait before harvesting my protein in the Expi293 Expression System?

The optimal expression time will be different for each protein, but most tested proteins fall within a 3-7 day window. As the system scales well, it is recommended you run a small-scale pilot experiment to determine when to harvest your protein of interest prior to scaling up.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

If I let my Expi293F cells grow to a higher density than recommended, will they be okay?

While the Expi293 Expression Medium can support much higher cell densities, we do not recommend growing your Expi293F cultures beyond 5-6 x 10e6 cells/mL, as subsequent transfection and protein expression efficiencies may be reduced. At higher densities, there is also the increased possibility of reaching the point of reduced culture viability. If your seed culture does exceed 5-6 x 10e6 cells/mL, passage them once or twice as detailed in the manual, monitoring them for viability and growth rate. Perform a test transfection using the Protein Expression Control IgG or an expression construct of known yield to determine if cell expression performance has been impacted.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Can I use a different 293 cell line with the Expi293 Expression System?

Other 293 cell lines may be used with the Expi293 Expression System. However, before these cell lines may be used for transfection studies, they must be adapted to serum-free, suspension culture in Expi293 Expression Medium and evaluated for transfection and expression.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Which vector do you recommend using with the Expi293 Expression System?

We recommend using the pcDNA 3.4-TOPO TA vector (Cat. No. A14697). This vector contains the native, full-length CMV promoter and a WPRE (Woodchuck Posttranscriptional Regulatory Element) downstream of the cloning site, both of which contribute to high level gene expression (about 2-3 fold higher expression than with pcDNA3.3 TOPO vector, which in turn provides 2-5 fold higher expression than with a standard pcDNA vector). Of course, the expression level is also protein-dependent.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all Expi293F™ Cells FAQs