pcDNA™3.4 TOPO™ TA Cloning Kit

본 제품은 LMO 제품으로, 고객 분께서 LMO 신고 시스템을 통해 직접 수입 신고를 진행해주셔야 합니다. 자세히보기

Invitrogen™

pcDNA™3.4 TOPO™ TA Cloning Kit

The pcDNA™ 3.4 TOPO™ vector is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.자세히 알아보기

카탈로그 번호	수량
A14697	20 μg Kit

카탈로그 번호 A14697

제품 가격(KRW)

1,665,000

카트에 추가하기

20 μg Kit

제품 가격(KRW)

1,665,000

카트에 추가하기

The pcDNA™ 3.4 TOPO™ vector is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression. It can be used in suspension-adapted cells, such as Expi293F™, FreeStyle™ 293-F, and FreeStyle™ CHO-S cells, for transient protein expression. The vector can also be used as a Geneticin™-selectable expression plasmid for engineering stable cell lines. The enhanced, full-length CMV promoter and other expression elements typically enable higher expression levels than pcDNA™ 3.1-, 3.2-, and 3.3-based expression constructs.

The pcDNA 3.4 TOPO™ vector contains the following elements:

• Full length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells
• WPRE (woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression
• TOPO™ cloning site for rapid and efficient (>85%) cloning of Taq-amplified PCR products
• Neomycin resistance gene for selection of stable cell lines with Geneticin™
• pUC origin for high copy replication and maintenance of the plasmid in E. coli

The pcDNA3.4™ TOPO™ TA Cloning Kit includes TOPO™-adapted plasmid vector, reagents for PCR cloning of your gene of choice into the vector, a sequencing primer for confirmation of insert orientation, and One Shot™ TOP10 competent cells.

For Research Use Only. Not for use in diagnostic procedures.

항생제 내성 박테리아Ampicillin (AmpR)

클로닝 방법TOPO™-TA

구성 또는 유도성 시스템Constitutive

배달 유형Transfection

용도(애플리케이션)Protein Production, Constitutive Expression

플라스미드High Copy Plasmid

제품라인TOPO

제품 유형TA Cloning Kit

프로모터CMV

단백질 태그Untagged

수량20 μg Kit

복제 개시점pUC Origin

선택 제제(진핵)Geneticin™ (G-418)

선택 마커 진핵NeoR

선택 마커 프로모터SV40 Promoter

벡터pcDNA

Unit SizeEach

구성 및 보관

Store pcDNA™3.4-TOPO™ vector and all cloning reagents at -20°C.
Store One Shot™ TOP10 Chemically Competent E. coli cells at -80°C.

자주 묻는 질문(FAQ)

Can I store my competent E. coli in liquid nitrogen?

We do not recommend storing competent E. coli strains in liquid nitrogen as the extreme temperature can be harmful to the cells. Also, the plastic storage vials are not intended to withstand the extreme temperature and may crack or break.

How should I store my competent E. coli?

We recommend storing our competent E. coli strains at -80°C. Storage at warmer temperatures, even for a brief period of time, will significantly decrease transformation efficiency.

Can I use a different 293 cell line with the Expi293 Expression System?

Other 293 cell lines may be used with the Expi293 Expression System. However, before these cell lines may be used for transfection studies, they must be adapted to serum-free, suspension culture in Expi293 Expression Medium and evaluated for transfection and expression.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the difference between pcDNA 3.4-TOPO TA and pcDNA 3.3-TOPO TA vectors?

pcDNA 3.4-TOPO TA vector is an improvement over pcDNA 3.3-TOPO TA vector. It contains the WPRE (Woodchuck Posttranscriptional Regulatory Element) that allows for 2- to 3-fold higher levels of expression than pcDNA 3.3-TOPO TA vector.

Which vector do you recommend using with the Expi293 Expression System?

We recommend using the pcDNA 3.4-TOPO TA vector (Cat. No. A14697). This vector contains the native, full-length CMV promoter and a WPRE (Woodchuck Posttranscriptional Regulatory Element) downstream of the cloning site, both of which contribute to high level gene expression (about 2-3 fold higher expression than with pcDNA3.3 TOPO vector, which in turn provides 2-5 fold higher expression than with a standard pcDNA vector). Of course, the expression level is also protein-dependent.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all pcDNA™3.4 TOPO™ TA Cloning Kit FAQs