This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4836:
The LanthaScreen™ TR-FRET Constitutive Androstane Receptor (CAR) Coactivator Assay provides a sensitive and robust method for highthroughput screening (HTS) of potential CAR ligands as agonists of ligand-dependent coactivator recruitment or inverse agonists of liganddependent coactivator displacement. The kit uses a terbium-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a CAR ligand-binding domain (CAR-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.
To assay: When running the LanthaScreen™ TR-FRET CAR Coactivator Assay, CAR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and terbium anti-GST antibody. After an incubation period at room temperature, the 520 nm/ 495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the CAR-coactivator peptide interaction, this ligand’s EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and either recruit or displace coactivator peptide (Figures 1 and 2).
For Research Use Only. Not for use in diagnostic procedures.
사양
색상
Green
콘주게이트
Tb (Terbium)
검출 방법
Fluorescent
용도(장비)
Microplate Reader
포장
384-well Plate
어세이 카테고리
Biochemical Assay
약효를 기대할 수 있는 타겟
Nuclear Receptors
판독
TR-FRET (Activity)
제품라인
LanthaScreen™
용도(애플리케이션)
Nuclear Receptor Assay, TR-FRET
리간드
CAR
제품 유형
TR-FRET CAR Coactivator Assay Kit
구성 및 보관
Tb-anti-GST Antibody (Rabbit): Store at -20 °C CAR-LBD, GST: Store at -80 °C Fluoroscein Coactivator Peptide: Store at -20 °C TR-FRET Coregulatore Buffer G: Store at 4 °C 1M DTT: Store at -20 °C or -80 °C