Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)

인용 및 참조 문헌 (101)

Invitrogen™

Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)

Name: Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)
SKU: A20006
Price: 624000 KRW

무이성질체, 아민 반응성인 Alexa Fluor 647 carboxylic acid, succinimidyl ester dye와 conjugates는 Cy5 dye와 분광학적으로 동일하여 여기/방출 최대값이 ∼650/668 nm입니다.자세히 알아보기

보기 방식 변경

카탈로그 번호	수량
A20006	1 mg
A37573	3 x 100 μg
A20106	5 mg
A37566	25 mg

4 개 옵션

카탈로그 번호 A20006

제품 가격(KRW)

624,000

キャンペーン価格

Ends: 31-Dec-2025

636,000

할인액 12,000 (2%)

Each

카트에 추가하기

수량:

1 mg

제품 가격(KRW)

624,000

キャンペーン価格

Ends: 31-Dec-2025

636,000

할인액 12,000 (2%)

Each

카트에 추가하기

무이성질체, 아민 반응성인 Alexa Fluor 647 carboxylic acid, succinimidyl ester dye와 conjugates는 Cy5 dye와 분광학적으로 동일하여 여기/방출 최대값이 ∼650/668 nm입니다. 하지만 Alexa Fluor 647 conjugate는 Cy5 dye로 준비된 것보다 훨씬 밝습니다. A 5 mg 단위(A-20106)도 구매 가능합니다. 장파장 Alexa Fluor dye의 형광은 인간 눈에는 보이지 않지만 대부분의 영상 시스템으로는 쉽게 검출이 가능합니다.

For Research Use Only. Not for use in diagnostic procedures.

사양

화학물질 반응성Amine

방출672 nm

여기651 nm

라벨 또는 염료Alexa Fluor™ 647

제품 유형NHS Ester (Succinimidyl Ester)

수량1 mg

반응성 부분Active Ester, Succinimidyl Ester

배송 조건Room Temperature

라벨 유형Alexa Fluor

제품라인Alexa Fluor

Unit SizeEach

구성 및 보관

Store in freezer (-5°C to -30°C) and protect from light.

자주 묻는 질문(FAQ)

I am labeling a protein with Alexa Fluor 488 SDP ester. The manual recommends using a sodium bicarbonate buffer at pH 8.3. Can I use a different buffer instead?

Yes. The important thing is to use a buffered solution with a pH between 8.0 and 8.5. Do not use Tris buffer, which has amine groups. Most other buffers will work fine in that pH range. This is also true for other amine-reactive dyes, such as succinimidyl (NHS) esters or TFP esters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am not going to use all of my Alexa Fluor succinimidyl ester reactive dye. Can I just make it up in DMSO and store aliquots at -20 degrees C?

This is not recommended. Any trace amounts of water in the DMSO can promote spontaneous hydrolysis over time. Even if using anhydrous DMSO, DMSO is hygroscopic; it readily absorbs moisture from the atmosphere over time. A better alternative is to dissolve the reactive dye in a volatile solvent, make smaller aliquots and then evaporate off the solvent using a vacuum pump. The smaller aliquots of solid reactive dye should then be stored frozen, desiccated and protected from light. Contact Technical Support by sending an email to techsupport@thermofisher.com for the recommended volatile solvent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (101)

인용 및 참조 문헌

Abstract

Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast.

Authors:Gupta R,Kus B,Fladd C,Wasmuth J,Tonikian R,Sidhu S,Krogan NJ,Parkinson J,Rotin D

Journal:Molecular systems biology

PubMed ID:17551511

Ubiquitin-protein ligases (E3s) are responsible for target recognition and regulate stability, localization or function of their substrates. However, the substrates of most E3 enzymes remain unknown. Here, we describe the development of a novel proteomic in vitro ubiquitination screen using a protein microarray platform that can be utilized for the ... More

Authors:

Journal:

PubMed ID:16263709

Surface plasmon field-enhanced fluorescence spectroscopy studies of the interaction between an antibody and its surface-coupled antigen.

Authors:Yu F, Yao D, Knoll W

Journal:Anal Chem

PubMed ID:12948127

Surface plasmon field-enhanced fluorescence spectroscopy (SPFS) uses the greatly enhanced electromagnetic field of a surface plasmon mode for the excitation of surface-confined fluorophores. The ability to simultaneously monitor the interfacial refractive index changes and the fluorescence signals in real time offers a huge potential for applications of SPFS in surface ... More

Niemann-Pick C1 functions in regulating lysosomal amine content.

Authors:Kaufmann AM, Krise JP,

Journal:J Biol Chem

PubMed ID:18591242

'Mutations in the late endosomal/lysosomal membrane protein Niemann-Pick C1 (NPC1) are known to cause a generalized block in retrograde vesicle-mediated transport, resulting in the hyper-accumulation of multiple lysosomal cargos. An important, yet often overlooked, category of lysosomal cargo includes the vast array of small molecular weight amine-containing molecules that are ... More

Death-receptor activation halts clathrin-dependent endocytosis.

Authors:Austin CD, Lawrence DA, Peden AA, Varfolomeev EE, Totpal K, De Mazière AM, Klumperman J, Arnott D, Pham V, Scheller RH, Ashkenazi A

Journal:Proc Natl Acad Sci U S A

PubMed ID:16801533

'Endocytosis is crucial for various aspects of cell homeostasis. Here, we show that proapoptotic death receptors (DRs) trigger selective destruction of the clathrin-dependent endocytosis machinery. DR stimulation induced rapid, caspase-mediated cleavage of key clathrin-pathway components, halting cellular uptake of the classic cargo protein transferrin. DR-proximal initiator caspases cleaved the clathrin ... More

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