Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)
인용 및 참조 문헌 (198)
Invitrogen™

Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)

amine 반응 Alexa Fluor 488 carboxylic acid, succinimidyl ester로 준비한 Conjugate는 fluorescein 보다 훨씬 밝고 광안정성이 높습니다. fluorescein과 분광학적으로 유사한자세히 알아보기
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카탈로그 번호수량
A201005 mg
A200001 mg
카탈로그 번호 A20100
제품 가격(KRW)
2,216,000
Online offer
Ends: 31-Mar-2026
2,606,000
할인액 390,000 (15%)
Each
카트에 추가하기
수량:
5 mg
제품 가격(KRW)
2,216,000
Online offer
Ends: 31-Mar-2026
2,606,000
할인액 390,000 (15%)
Each
카트에 추가하기
amine 반응 Alexa Fluor 488 carboxylic acid, succinimidyl ester로 준비한 Conjugate는 fluorescein 보다 훨씬 밝고 광안정성이 높습니다. fluorescein과 분광학적으로 유사한 Alexa Fluor 488 dye의 밝은 녹색 형광은 pH 4-10에서 pH 의존성을 보입니다. 5 mg 단위(A-20100)도 구매 가능합니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
화학물질 반응성Amine
방출517 nm
여기494 nm
라벨 또는 염료Alexa Fluor™ 488
제품 유형Dye
수량5 mg
반응성 부분Active Ester, TFP Ester
배송 조건Room Temperature
라벨 유형Alexa Fluor
제품라인Alexa Fluor
Unit SizeEach
구성 및 보관
Store in freezer (-5 to -30°C) and protect from light.

자주 묻는 질문(FAQ)

I am labeling a protein with Alexa Fluor 488 SDP ester. The manual recommends using a sodium bicarbonate buffer at pH 8.3. Can I use a different buffer instead?

Yes. The important thing is to use a buffered solution with a pH between 8.0 and 8.5. Do not use Tris buffer, which has amine groups. Most other buffers will work fine in that pH range. This is also true for other amine-reactive dyes, such as succinimidyl (NHS) esters or TFP esters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am not going to use all of my Alexa Fluor succinimidyl ester reactive dye. Can I just make it up in DMSO and store aliquots at -20 degrees C?

This is not recommended. Any trace amounts of water in the DMSO can promote spontaneous hydrolysis over time. Even if using anhydrous DMSO, DMSO is hygroscopic; it readily absorbs moisture from the atmosphere over time. A better alternative is to dissolve the reactive dye in a volatile solvent, make smaller aliquots and then evaporate off the solvent using a vacuum pump. The smaller aliquots of solid reactive dye should then be stored frozen, desiccated and protected from light. Contact Technical Support by sending an email to techsupport@thermofisher.com for the recommended volatile solvent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the signal intensity differences between Alexa Fluor 350 dye and Alexa Fluor 488 dye?

In general, blue fluorescent dyes are not as bright as other dyes further along the color spectrum. Blue dyes are structurally smaller and have lower extinction coefficients, so they are typically not as bright compared to the green, red, and far red dyes.
When using an Alexa Fluor 350 secondary antibody, we recommend that you use it for highly expressed targets and at a higher concentration than what is typically required for green or red secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (198)

인용 및 참조 문헌
Abstract
Activity of human IgG and IgA subclasses in immune defense against Neisseria meningitidis serogroup B.
Authors:Vidarsson G,van Der Pol WL,van Den Elsen JM,Vilé H,Jansen M,Duijs J,Morton HC,Boel E,Daha MR,Corthésy B,van De Winkel JG
Journal:Journal of immunology (Baltimore, Md. : 1950)
PubMed ID:11342648
Reconstituted syntaxin1a/SNAP25 interacts with negatively charged lipids as measured by lateral diffusion in planar supported bilayers.
Authors:Wagner ML, Tamm LK
Journal:Biophys J
PubMed ID:11423412
According to the soluble N-ethylmaleimide-sensitive factor (NSF)-attachment protein (SNAP) receptor hypothesis (SNARE hypothesis), interactions between target SNAREs and vesicle SNAREs (t- and v-SNAREs) are required for membrane fusion in intracellular vesicle transport and exocytosis. The precise role of the SNAREs in tethering, docking, and fusion is still disputed. Biophysical measurements ... More
Tubulin equilibrium unfolding followed by time-resolved fluorescence and fluorescence correlation spectroscopy.
Authors:Sánchez SA, Brunet JE, Jameson DM, Lagos R, Monasterio O
Journal:Protein Sci
PubMed ID:14691224
The pathway for the in vitro equilibrium unfolding of the tubulin heterodimer by guanidinium chloride (GdmCl) has been studied using several spectroscopic techniques, specifically circular dichroism (CD), two-photon Fluorescence Correlation Spectroscopy (FCS), and time-resolved fluorescence, including lifetime and dynamic polarization. The results show that tubulin unfolding is characterized by distinct ... More
Segregation of nitrogen fixation and oxygenic photosynthesis in the marine cyanobacterium Trichodesmium.
Authors:Berman-Frank I, Lundgren P, Chen YB, Küpper H, Kolber Z, Bergman B, Falkowski P
Journal:Science
PubMed ID:11711677
'In the modern ocean, a significant amount of nitrogen fixation is attributed to filamentous, nonheterocystous cyanobacteria of the genus Trichodesmium. In these organisms, nitrogen fixation is confined to the photoperiod and occurs simultaneously with oxygenic photosynthesis. Nitrogenase, the enzyme responsible for biological N2 fixation, is irreversibly inhibited by oxygen in ... More
Cytoplasmic dynein/dynactin drives kinetochore protein transport to the spindle poles and has a role in mitotic spindle checkpoint inactivation.
Authors:Howell BJ, McEwen BF, Canman JC, Hoffman DB, Farrar EM, Rieder CL, Salmon ED
Journal:J Cell Biol
PubMed ID:11756470
'We discovered that many proteins located in the kinetochore outer domain, but not the inner core, are depleted from kinetochores and accumulate at spindle poles when ATP production is suppressed in PtK1 cells, and that microtubule depolymerization inhibits this process. These proteins include the microtubule motors CENP-E and cytoplasmic dynein, ... More
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